The 5S rDNA High Dynamism in Diplodus sargus is a Transposon-Mediated Mechanism. Comparison with Other Multigene Families and Sparidae Species

• Published in: Journal of molecular evolution Vol. 76; no. 3; pp. 83 - 97
• Main Authors: Merlo, Manuel A., Cross, Ismael, Manchado, Manuel, Cárdenas, Salvador, Rebordinos, Laureana
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.03.2013; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Animals; Biomedical and Life Sciences; Cell Biology; Diplodus sargus; DNA Transposable Elements - physiology; DNA, Ribosomal - genetics; Evolution & development; Evolution, Molecular; Evolutionary Biology; Female; Fish; Gene Expression Regulation, Developmental; Genetic Speciation; Genetic Variation - physiology; Genetics; Life Sciences; Male; Microbiology; Multigene Family - genetics; Pagrus; Pagrus pagrus; Perciformes - classification; Perciformes - genetics; Phylogeny; Plant Genetics and Genomics; Plant Sciences; RNA, Ribosomal, 5S - genetics; Sargus; Sparidae; Species Specificity; Taxonomy; Birth-and-death evolution; Multigene family; Hybrids; Sparidae; Concerted evolution
• ISSN: 0022-2844; 1432-1432; 1432-1432
• DOI: 10.1007/s00239-013-9541-8

There has been considerable discussion in recent years on the evolution of the tandemly repeated multigene families, since some organisms show a concerted model whereas others show a birth-and-death model. This controversial subject extends to several species of fish. In this study, three species of the Sparidae family ( Pagrus pagrus , P. auriga and Diplodus sargus ) and an interspecific hybrid ( P. pagrus (♀) ×  P. auriga (♂)) have been studied at both molecular and cytogenetic level, taking three different multigene families (5S rDNA, 45S rDNA and U2 snDNA). Results obtained with the 5S rDNA in P. pagrus and P. auriga are characterized by a considerable degree of conservation at the two levels; however, an extraordinary variation was observed in D. sargus at the two levels, which has never been found in other fishes studied to date. As a consequence of this, the evolutionary model of the multigene families is discussed considering the results obtained and others from the bibliography. The result obtained in the hybrid allowed the recombination frequency in each multigene family to be estimated.