Fluorescent probe based subcellular distribution of Cu(II) ions in living electrotrophs isolated from Cu(II)-reduced biocathodes of microbial fuel cells

• Published in: Bioresource technology Vol. 225; pp. 316 - 325
• Main Authors: Tao, Ye, Xue, Hua, Huang, Liping, Zhou, Peng, Yang, Wei, Quan, Xie, Yuan, Jinxiu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2017
• Subjects: Biocathode; Bioelectric Energy Sources; Copper - analysis; Copper - chemistry; Copper - metabolism; Cu(II) recovery; Electrodes; Electrons; Electrotroph; Fluorescent Dyes - analysis; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Gram-Negative Bacteria - chemistry; Gram-Negative Bacteria - metabolism; Intracellular Space - chemistry; Intracellular Space - metabolism; Microbial fuel cell; Rhodamine based Cu(II) fluorescent probe; Rhodamines - analysis; Rhodamines - chemistry; Rhodamines - metabolism; Microbial fuel cell; Biocathode; Cu(II) recovery; Electrotroph; Rhodamine based Cu(II) fluorescent probe
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.11.084

[Display omitted] •Electrotrophs were isolated from Cu(II)-reduced biocathodes of microbial fuel cells.•Cu(II) removal rates of 2.90–3.64mg/Lh were achieved by these electrotrophs.•Fluorescent probe was used to successfully track Cu(II) ions in electrotrophs.•Cathodic electrons directed more Cu(II) ions for quicker entrance into cytoplasm. Based on the four indigenous electrotrophs (Stenotrophomonas maltophilia JY1, Citrobacter sp. JY3, Pseudomonas aeruginosa JY5 and Stenotrophomonas sp. JY6) isolated from well adapted Cu(II)-reduced biocathodes of microbial fuel cells (MFCs), a rhodamine based Cu(II) fluorescent probe was used to imaginably and quantitatively track subcellular Cu(II) ions in these electrotrophs. Cathodic electrons led to more Cu(II) ions (14.3–30.1%) in the intracellular sites at operation time of 2–3h with Cu(II) removal rates of 2.90–3.64mg/Lh whereas the absence of cathodic electrons prolonged the appearance of more Cu(II) ions (16.6–22.5%) to 5h with Cu(II) removal rates of 1.96–2.28mg/Lh. This study illustrates that cathodic electrons directed more Cu(II) ions for quicker entrance into the electrotrophic cytoplasm, and gives an alternative approach for developing imaging and functionally tracking Cu(II) ions in the electrotrophs of MFCs.