Alterations in serum electrolytes, antioxidative enzymes and haematological parameters of Labeo rohita on short-term exposure to sublethal dose of nitrite

• Published in: Fish physiology and biochemistry Vol. 38; no. 5; pp. 1355 - 1365
• Main Authors: Ciji, Alexander, Sahu, N. P., Pal, A. K., Dasgupta, S., Akhtar, M. S.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.10.2012; Springer Nature B.V
• Subjects: Acetylcholinesterase - metabolism; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Antioxidants - metabolism; Aquaculture; Biomedical and Life Sciences; Blood; Blood Glucose; Catalase - metabolism; Cyprinidae - blood; Cyprinidae - metabolism; Cytochrome-B Reductase - metabolism; Dose-Response Relationship, Drug; Drug Administration Schedule; Electrolytes; Electrolytes - blood; Enzymes; Exposure; Fish; Fisheries; Freshwater & Marine Ecology; Gene Expression Regulation, Enzymologic - drug effects; Hematology; Hemoglobin; Histology; Hormones; Hydrocortisone - blood; Labeo rohita; Lethal Dose 50; Life Sciences; Metabolites; Morphology; Nitrates; Nitrites - administration & dosage; Nitrites - toxicity; Physiology; Potassium; Sodium; Superoxide Dismutase - metabolism; Toxicity; Zoology; India; Electrolytes; Cortisol; Nitrite; Methaemoglobin reductase
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-012-9623-3

An experiment was conducted to study the effects of short-term exposure to sublethal levels of nitrite on electrolyte regulation, antioxidative enzymes and haematological parameters in Labeo rohita juveniles. The fishes were exposed to graded levels of nitrite (0–15 mg l −1 ) for different duration (0, 12, 24, 48 and 96 h). The 96-h LC 50 value for L. rohita (avg. wt, 66.5 ± 0.5 g) was found to be 11.28 mg l −1 . Activities of antioxidative enzymes (catalase and superoxide dismutase), acetylcholine esterase (AChE) and methaemoglobin reductase, serum electrolytes (sodium, potassium and chloride), haematological parameters and blood glucose level significantly varied ( P  < 0.05) in a dose-dependent manner. With increasing nitrite concentration and exposure period, a progressive reduction in the total erythrocyte count and haemoglobin were observed. With increase in nitrite concentration, a significant ( P  < 0.05) increase in activities was evidenced in catalase and superoxide dismutase in liver as well as gill, methaemoglobin reductase in blood, while progressive decline in AChE activity in brain was recorded. The serum sodium and chloride content showed a progressive decline, while potassium showed an increasing trend upon increase in nitrite concentration. The serum K + and Cl − after 96-h exposure demonstrated a linear relationship (Y = 0.221 x  + 2.542, R 2  = 0.938, P  < 0.01 and Y = −5.760 x  + 129.5, R 2  = 0.952, P  < 0.01, respectively) with nitrite concentrations. This study revealed that nitrite exposure causes alteration in all measured tissue enzymes, serum electrolytes and haematological parameters.