Screening 34 Peanut Introductions for Allergen Content Using ELISA

Peanut is one of the most allergenic foods and reports of accidental ingestion of peanuts in unsuspected food are increasing. No information is available on the allergen content of peanut germplasm grown commercially and used in the food and confectionery industry. The objectives of this study were:...

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Bibliographic Details
Published inFood and agricultural immunology Vol. 14; no. 2; pp. 147 - 154
Main Authors Dodo, Hortense, Marsic, Damien, Callender, Monique, Cebert, Ernst, Viquez, Olga
Format Journal Article
LanguageEnglish
Published London Taylor & Francis Group 01.06.2002
Taylor and Francis
Subjects
Allergen
Biological and medical sciences
Confectionery products and chocolate industries, honey
Elisa
Food industries
Fundamental and applied biological sciences. Psychology
Human
Hypo-ALLERGENIC
Introduction
Peanut
Plant
Serum
South America
America
Peanut
Nuts
Genetic variability
Arachis hypogaea
Geographical origin
Enzyme immunoassay
Allergenicity
Screening
Germplasm
Leguminosae
Dicotyledones
Angiospermae
Spermatophyta
ELISA assay
Oil plant (vegetal)
Allergen
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Summary:Peanut is one of the most allergenic foods and reports of accidental ingestion of peanuts in unsuspected food are increasing. No information is available on the allergen content of peanut germplasm grown commercially and used in the food and confectionery industry. The objectives of this study were: (1) to determine the allergen contents of 34 peanut introductions (PI); and (2) to identify naturally occurring allergen-free and/or low or hypoallergenic peanuts germplasm. A basic ELISA protocol was utilized to detect the presence of antigens in the peanut lines using a pool of human sera from patients with documented history of peanut allergy. Two naturally occurring low, or hypo-allergenic germplasm were identified as PI 261942 and PI 338386. Both are Valencia market types with total allergen content significantly lower (P ≤0.05) than that of PI 119880 (0.550), PI 119876 (0.415) and PI 118991 (0.410) three Valencia market types and PI 262111 (0.485), a Virginia market type. No allergen-free PI was found. Allergen content of peanut lines from Bolivia and Paraguay were significantly (P ≤0.05) different to those from Venezuela. No significant difference was observed in the allergen content of the four market types.
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ISSN:0954-0105
1465-3443
DOI:10.1080/09540100220145179