Newly Isolated Lactic Acid Bacteria with Probiotic Features for Potential Application in Food Industry
Five newly isolated lactic acid bacteria were identified as Weissella cibaria , Enterococcus faecium , and three different strains of Lactobacillus plantarum by 16S rRNA sequencing. Essential probiotic requirements of these isolates such as tolerance to phenol, low pH, high sodium chloride, and bile...
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Published in | Applied biochemistry and biotechnology Vol. 167; no. 5; pp. 1314 - 1324 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer-Verlag
01.07.2012
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Five newly isolated lactic acid bacteria were identified as
Weissella cibaria
,
Enterococcus faecium
, and three different strains of
Lactobacillus plantarum
by 16S rRNA sequencing. Essential probiotic requirements of these isolates such as tolerance to phenol, low pH, high sodium chloride, and bile salt concentration were checked. Efficiency in adherence to mucin and hydrophobicity of the bacterial cell were also evaluated by in vitro studies. Antimicrobial activities against some pathogens were tried, and the sensitivity of these strains against 25 different antibiotics was also checked. Further studies revealed
Weissella
and
Enterococcus
as substantial producers of folic acid. Folate is involved as a cofactor in many metabolic reactions, and it has to be an essential component in the human diet. The folate level in the fermented samples was determined by microbiological assay using
Lactobacillus casei
NCIM 2364 as indicator strain. The three strains of
L. plantarum
showed significant inhibitory activity against various fungi that commonly contaminate food stuffs indicating their potential as a biopreservative of food material. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0273-2289 1559-0291 |
DOI: | 10.1007/s12010-012-9561-7 |