Atomic Force Microscopy Studies of Living Cells: Visualization of Motility, Division, Aggregation, Transformation, and Apoptosis

Atomic force microscopy, in contact mode, has been used to image living mammalian cells in culture at both low and high resolutions. The method is shown practical for revealing cytoskeletal features beneath the cell membrane and their restructuring during a variety of cellular activities. Among the...

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Published inJournal of structural biology Vol. 120; no. 2; pp. 180 - 191
Main Authors Kuznetsov, Yurii G., Malkin, Alexander J., McPherson, Alexander
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.1997
Subjects
Animals
Apoptosis
Cell Aggregation
Cell Division
cell interactions
Cell Movement
Cell Transformation, Viral
Cells, Cultured
Chick Embryo
cytoskeleton
Cytoskeleton - ultrastructure
Fibroblasts - ultrastructure
Humans
Mice
Microscopy, Atomic Force
Osteoblasts - ultrastructure
Osteosarcoma - ultrastructure
scanning probe
Space life sciences
cell interactions
cytoskeleton
scanning probe
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Summary:Atomic force microscopy, in contact mode, has been used to image living mammalian cells in culture at both low and high resolutions. The method is shown practical for revealing cytoskeletal features beneath the cell membrane and their restructuring during a variety of cellular activities. Among the processes that we have visualized are locomotion, tissue formation, cell division, transformation by viruses, and cell death. We show that some processes that occur well within cells can, nonetheless, be observed using the atomic force probe. At high resolution, features on the cell surface on the order of 0.5 μm, and their changes with time, can be recorded.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1047-8477
1095-8657
DOI:10.1006/jsbi.1997.3936