Chemotactic, mitogenic, and angiogenic actions of UTP on vascular endothelial cells
Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557 Endothelial cells express receptors for ATP and UTP, and both UTP and ATP elicit endothelial release of vasoactive compounds such as prostacyclin and nitric oxide; however, the distinction between purine and pyri...
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Published in | American journal of physiology. Heart and circulatory physiology Vol. 276; no. 3; pp. H1091 - H1097 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.03.1999
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Subjects | |
Online Access | Get full text |
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Summary: | Department of Pharmacology, University of Nevada School of Medicine,
Reno, Nevada 89557
Endothelial
cells express receptors for ATP and UTP, and both UTP and ATP elicit
endothelial release of vasoactive compounds such as prostacyclin and
nitric oxide; however, the distinction between purine and pyrimidine
nucleotide signaling is not known. We hypothesized that UTP plays a
more important role in endothelial mitogenesis and chemotaxis than does
ATP and that UTP is angiogenic. In cultured endothelial cells from
guinea pig cardiac vasculature (CEC), both UTP and vascular endothelial
growth factor (VEGF) were significant mitogenic and chemotactic
factors; in contrast, ATP demonstrated no significant chemotaxis in
CEC. In chick chorioallantoic membranes (CAM), UTP and VEGF treatments
produced statistically significant increases in CAM vascularity
compared with controls. These findings are the first evidence of
chemotactic or angiogenic effects of pyrimidines; they suggest a role
for pyrimidine nucleotides that is distinct from those assumed by
purine nucleotides and provide for the possibility that UTP serves
as an extracellular signal for processes such as endothelial repair and angiogenesis.
uridine 5'-triphosphate; chemotaxis; angiogenesis; mitogenesis |
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ISSN: | 0363-6135 0002-9513 1522-1539 |
DOI: | 10.1152/ajpheart.1999.276.3.h1091 |