Electrodeposition to construct free-standing chitosan/layered double hydroxides hydro-membrane for electrically triggered protein release

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 158; pp. 474 - 479
• Main Authors: Zhao, Pengkun, Zhao, Yanan, Xiao, Ling, Deng, Hongbing, Du, Yumin, Chen, Yun, Shi, Xiaowen
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2017
• Subjects: Chitosan; Chitosan - chemistry; Controlled drug release; Electrodeposition; Electroplating - methods; Hydro-membrane; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry; Hydroxides - chemistry; LDHs; X-Ray Diffraction; LDHs; Electrodeposition; Hydro-membrane; Controlled drug release; Chitosan
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2017.07.024

[Display omitted] •Free-standing chitosan/LDHs hydro-membrane was co-electrodeposited in the absence of salt.•The chitosan/LDHs hydro-membrane was mechanically strong and had multilayered structure.•Electrical signals were used to trigger insulin release from the chitosan/LDHs hydro-membrane. In this study, we report the electrodeposition of a chitosan/layered double hydroxides (LDHs) hydro-membrane for protein release triggered by an electrical signal. The electrodeposition was performed in a chitosan and insulin loaded LDHs suspension in the absence of salt. A free-standing chitosan/LDHs hydro-membrane was generated on the electrode with improved mechanical properties, which is dramatically different from the weak hydrogel deposited in the presence of salt. The amount of LDHs in the hydro-membrane affects the optical transmittance and multilayered structure of the hybrid membrane. Compared to the weak chitosan/LDHs hydrogel, the hydro-membrane has a higher insulin loading capacity and the release of insulin is relatively slow. By biasing electrical potentials to the hydro-membrane, the release behavior of insulin can be adjusted accordingly. In addition, the chitosan/LDHs hydro-membrane showed no toxicity to cells. Our results provide a facile method to construct a chitosan/LDHs hybrid multilayered hydro-membrane and suggest the great potential of the hydro-membrane in controlled protein release.