Feruloyl esterases from Schizophyllum commune to treat food industry side-streams

• Published in: Bioresource technology Vol. 220; pp. 38 - 46
• Main Authors: Nieter, Annabel, Kelle, Sebastian, Linke, Diana, Berger, Ralf G.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2016
• Subjects: Agro-industrial side-streams; Basidiomycota; Benzoic Acid - chemistry; Benzoic Acid - metabolism; Benzoic acid ester; Biotechnology - methods; Carbohydrate Metabolism; Carboxylic Ester Hydrolases - genetics; Carboxylic Ester Hydrolases - metabolism; Coffee - chemistry; Coumaric Acids - metabolism; Dietary Fiber - metabolism; Feruloyl esterase; Food Industry; Hydrolysis; Industrial Waste; Pectins - chemistry; Pectins - metabolism; Pichia - genetics; Pichia - metabolism; Pichia pastoris; Propionates; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Schizophyllum - enzymology; Schizophyllum - genetics; Substrate Specificity; Agro-industrial side-streams; Feruloyl esterase; Benzoic acid ester; Pichia pastoris; Basidiomycota
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.08.045

•Identification of the first type D feruloyl esterases from Basidiomycota.•Functional production of Schizophyllum commune feruloyl esterases in Pichia pastoris.•The enzymes possessed unique substrate profiles and hydrolyzed benzoic acid esters.•The enzymes preferred substrates typical of graminaceous monocots.•Hydroxycinnamic acids were released from complex side-streams of food processing. Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000UL−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.