Preparation and characterization of medium-chain fatty acid liposomes by lyophilization

• Published in: Journal of liposome research Vol. 20; no. 3; pp. 183 - 190
• Main Authors: Liu, Chengmei, Yang, Shuibing, Liu, Wei, Wang, Ruilian, Wan, Jie, Liu, Weilin
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.09.2010; Taylor & Francis
• Subjects: Caprylates - chemistry; Chemical Phenomena; Decanoic Acids - chemistry; entrapment efficiency; Freeze Drying - methods; liposomes; Liposomes - chemical synthesis; Liposomes - chemistry; lyophilization; Medium chain fatty acid; Microscopy, Electron, Transmission
• ISSN: 0898-2104; 1532-2394
• DOI: 10.3109/08982100903244500

In this study, medium-chain fatty acid (MCFA) liposomes were prepared by the film ultrasonic dispersion, modified ethanol injection, and reverse-phase evaporate methods. The results indicated that the liposomes prepared by the thin-film ultrasonic dispersion method had a high entrapment efficiency of 82.7% and a good distribution in size diameters. The MCFA liposomes were freeze-dried and the optimal preparation conditions of freeze-drying were as follows: The cryoprotectants were mannitol and sucrose (1:1 w/w), the hydrated medium was distilled water, and the freeze-drying time was 48 hours. Under these conditions, the freeze-dried MCFA liposomes had a perfect appearance, a small particle size, and high encapsulation efficiency. The mean diameters were 251.1 and 265.3 nm, and the encapsulation efficiencies were 80.5 and 79.2% for freshly prepared and reconstituted liposomes, respectively.