Span® and Tween® neutral and pH-sensitive vesicles: Characterization and in vitro skin permeation

The aim of this work was the preparation, characterization, and comparison of novel pH-sensitive nonphospholipid vesicles (niosomes) from two nonionic surfactants, with different hydrophilic-lipophilic balance values (Tween®20-TW20 = 16.7 and Span®60-SP60 = 4.7). Surfactants were mixed with choleste...

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Published inJournal of liposome research Vol. 19; no. 4; pp. 332 - 340
Main Authors Carafa, Maria, Marianecci, Carlotta, Rinaldi, Federica, Santucci, Eleonora, Tampucci, Silvia, Monti, Daniela
Format Journal Article
LanguageEnglish
Published England Informa UK Ltd 01.12.2009
Taylor & Francis
Subjects
Animals
Chromatography, High Pressure Liquid
Fluorescence
Freeze Fracturing
Hexoses - chemistry
Hydrogen-Ion Concentration
ibuprofen
In Vitro Techniques
Permeability
pH-sensitive surfactant vesicles
Polysorbates - chemistry
Rats
Rats, Hairless
Skin Absorption
skin permeation
Span
Surface-Active Agents - chemistry
Tween
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Summary:The aim of this work was the preparation, characterization, and comparison of novel pH-sensitive nonphospholipid vesicles (niosomes) from two nonionic surfactants, with different hydrophilic-lipophilic balance values (Tween®20-TW20 = 16.7 and Span®60-SP60 = 4.7). Surfactants were mixed with cholesterol (CHOL) and its derivative, cholesteryl hemisuccinate (CHEMS), as a pH-sensitive molecule. Vesicles were characterized by dynamic light scattering, in order to evaluate their dimensions and vesicle stability, by ζ-potential measurements and by means of electronic microscopy after freeze-fracture. Ibuprofen (IBU) was used as the model drug, and high-performance liquid chromatography analyses were performed to evaluate drug-entrapment efficiency and release in a neutral, acidic environment. The influence of the vesicle composition on skin accumulation and transdermal permeation of IBU across excised hairless rat skin was investigated by using vertical Gummer diffusion cells. When niosomes with SP60 and CHEMS were prepared, there was a statistically significant increase of skin permeation of IBU, while TW20 niosomes did not show statistically significant differences in Papp values without the influence of the vesicle size and charge.
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ISSN:0898-2104
1532-2394
DOI:10.3109/08982100903014994