Culture-induced variation in plants of Coffea arabica cv. caturra rojo, regenerated by direct and indirect somatic embryogenesis

• Published in: Molecular biotechnology Vol. 23; no. 2; pp. 107 - 115
• Main Authors: SANCHEZ-TEYER, L. Felipe, QUIROZ-FIGUEROA, Francisco, LOYOLA-VARGAS, Victor, INFANTE, Diogenes
• Format: Journal Article
• Language: English
• Published: Totowa, NJ Humana Press 01.02.2003; Springer Nature B.V
• Subjects: Biological and medical sciences; Biotechnology; Breeding - methods; Cloning, Organism - methods; Cluster analysis; Coffea - embryology; Coffea - genetics; Coffea - growth & development; Coffea arabica; Culture Techniques - methods; Deoxyribonucleic acid; DNA; DNA Fingerprinting - methods; DNA, Plant - genetics; Embryonic growth stage; Embryos; Eukaryotic cell cultures; Flowers & plants; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - methods; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genetic Variation; In vitro propagation: entire plant regeneration from tissues and cell cultures; Methods. Procedures. Technologies; Plant cells and fungal cells; Plant Leaves - embryology; Plant Leaves - genetics; Plant Leaves - growth & development; Polymorphism, Restriction Fragment Length; Random Amplified Polymorphic DNA Technique - methods; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology; Stability; Coffea arabica; genetic variation; bulk analysis; Somatic embryo; Regeneration; Gene amplification; Breeding line; Dicotyledones; DNA; Angiospermae; Rubiaceae; AFLP; Spermatophyta; cluster analysis; Amplified fragment length polymorphism marker; molecular markers; Polymorphism
• ISSN: 1073-6085; 1559-0305; 1073-6085
• DOI: 10.1385/MB:23:2:107

Amplified fragment-length polymorphism (AFLP) was used to evaluate the stability of DNA in regenerated plantlets of Coffea arabica obtained by direct (DSE) and indirect somatic embryogenesis (ISE). Cluster analysis using the unweighted pair-group method (UPGMA), showed no specific grouping pattern related to the type of embryogenesis. These results suggest that the somatic embryogenesis (SE) process has a mechanism for the selection of normal and competent cells. Bulked DNA from regenerated plants obtained by DSE and ISE, and from the mother plants, was used to characterize specific AFLP fragments associated with each SE process. Twenty-three primer combinations were tested. A total of 1446 bands were analyzed, with 11.4% being polymorphic and 84% being specific for regenerated plants. Furthermore, specific bands were detected for DSE, ISE, and the mother plants. These results indicate that the SE process induces rearrangements at the DNA level and demonstrates discrepancies between the mechanisms involved in each SE process. Coffea arabica breeding programs that involve DSE and ISE can use AFLP as an additional tool for assessing DNA stability.