cDNA Cloning and Genomic Structure of Human Bone Morphogenetic Protein-3b (BMP-3b)

• Published in: Biochemical and biophysical research communications Vol. 223; no. 2; pp. 304 - 310
• Main Authors: Hino, Jun, Takao, Makoto, Takeshita, Norimatsu, Konno, Yasuhiko, Nishizawa, Tsutomu, Matsuo, Hisayuki, Kangawa, Kenji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.06.1996
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Bone and Bones - metabolism; Bone Morphogenetic Protein 3; Bone Morphogenetic Proteins; Brain - metabolism; Chromosome Mapping; Chromosomes, Human, Pair 10; Cloning, Molecular; DNA Primers; DNA, Complementary; Exons; Growth Differentiation Factor 10; Growth Substances - biosynthesis; Growth Substances - genetics; Humans; Lung - metabolism; Male; Molecular Sequence Data; Muscle, Skeletal - metabolism; Pancreas - metabolism; Polymerase Chain Reaction; Rats; Regulatory Sequences, Nucleic Acid; Restriction Mapping; RNA, Messenger - biosynthesis; TATA Box; Testis - metabolism; Transcription, Genetic
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1996.0889

BMP-3b (also termed GDF-10) is a novel BMP-3 related protein recently discovered in rat femur tissue by molecular cloning. In this paper, we have isolated cDNA and the gene for human BMP-3b and determined their structure. Cloned human BMP-3b cDNA with a size of 2632 base pairs encodes a 478 amino acid precursor protein sharing 83% identity with rat BMP-3b. The human BMP-3b gene is composed of 3 exons and spans approximately 13 kilobases of DNA. The 5′-flanking region carries no typical TATA box but G+C rich sequences. Southern blot analysis revealed that the BMP-3b gene is situated in a single locus of chromosome 10. By Northern analysis, human BMP-3b transcripts were detected primarily in femur, brain, lung, skeletal muscle, pancreas and testis.