Characterization of a Specific Monoclonal-Antibody 9F5-3a and the Development of Assay System for Oxidized HDL

• Published in: Biochemical and biophysical research communications Vol. 217; no. 2; pp. 407 - 411
• Main Authors: Nakajima, T., Sakagishi, Y., Katahira, T., Nagata, A., Kuwae, T., Nakamura, H., Inoue, I., Takahashi, K., Katayama, S., Komoda, T.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.12.1995
• Subjects: Animals; Antibodies, Monoclonal - immunology; Arteriosclerosis - etiology; Copper - chemistry; Copper Sulfate; Lipoproteins, HDL - chemistry; Lipoproteins, HDL - immunology; Lipoproteins, LDL - chemistry; Lipoproteins, LDL - immunology; Lysophosphatidylcholines - immunology; Mice; Mice, Inbred BALB C; Oxidation-Reduction
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1995.2791

We obtained a monoclonal antibody 9F5-3a against oxidative low-density lipoprotein (LDL) modified with CuSO4 and established a sandwich ELISA for detection of oxidized high-density lipoprotein (oxHDL). The 9F5-3a was reacted strongly with oxHDL and to a lesser degree with oxLDL and LDL. In contrast, little or no reactivity was found with HDL. When the generation of oxHDL was limited by the addition of α-tocopherol and catalase, reactivity to 9F5-3a was reduced. Incubation of oxHDL with excess lyso-phosphatidylcholine (lyso-PC) also reduced immunoreactivity, but not by only lyso-PC. These results suggested that the epitope is possibly associated with oxHDL-linked lyso-PC induced mainly by the hydroxyl radical.