Untargeted metabolite profiling and phytochemical analysis of Micromeria fruticosa L. (Lamiaceae) leaves

• Published in: Food chemistry Vol. 279; pp. 128 - 143
• Main Authors: Abu-Reidah, Ibrahim M., Arráez-Román, David, Al-Nuri, Mohammed, Warad, Ismail, Segura-Carretero, Antonio
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2019
• Subjects: Chromatography, High Pressure Liquid; Flavonoids; Lamiaceae; Lamiaceae - chemistry; Lamiaceae - metabolism; Micromeria fruticosa; Phenolics; Phenols - analysis; Phenols - chemistry; Phytochemicals - analysis; Phytochemicals - chemistry; Plant Extracts - chemistry; Plant Leaves - chemistry; Plant Leaves - metabolism; Secondary metabolites; Spectrometry, Mass, Electrospray Ionization - methods; Sulfate derivatives; Ultra-high-performance liquid chromatography-diode array detection-electrospray ionization-quadrupole time of flight-mass spectrometry (UHPLC-DAD-ESI-QTOF-MS2); Ultra-high-performance liquid chromatography-diode array detection-electrospray ionization-quadrupole time of flight-mass spectrometry (UHPLC-DAD-ESI-QTOF-MS2); Rutin (PubChem CID: 5280805); Acacetin-7-O-rutinoside (PubChem CID: 5317025); Micromeria fruticosa; Chlorogenic acid (PubChem CID: 1794427); Coumaroylagmatine (PubChem CID: 5280691); Lamiaceae; Phenolics; Lithospermic acid (PubChem CID: 6441498); Salvinorin C (PubChem CID: 11134539); Apigenin 7-O-rutinoside (PubChem CID: 9851181); Secondary metabolites; Flavonoids; Santaflavone (PubChem CID: 44258695); Rosmarinic acid (PubChem CID: 5281792); Sulfate derivatives; Sagerinic acid (PubChem CID: 23760102); Ultra-high-performance liquid chromatography-diode array detection-electrospray ionization-quadrupole time of flight-mass spectrometry (UHPLC-DAD-ESI-QTOF-MS)
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2018.11.144

[Display omitted] •Metabolites from Palestinian Micromeria fruticosa was reported for the first time.•Over 200 components were detected and tentatively identified by UHPLC-DAD-MS2 method.•M. fruticosa showed to be a natural source of phytochemical and functional ingredients.•Sulfate derivatives are unusual components newly identified in the Micromeria genus. Micromeria fruticosa is an important crop, is widely used in the Mediterranean basin as food and in folk medicine, owing to its health-promoting properties, partially due to the secondary metabolite composition. However, complete information on the phyto-metabolites in M. fruticosa is still lacking. Plant leaves were extracted in methanol (80%), then the phyto-metabolites were separated on C18 column and an extensive characterization using UHPLC–DAD-ESI–QTOF–MS2 method in two ionization modes was established. A total of 215 phenolics and other compounds were tentatively identified, offering the first comprehensive study available on the phytochemicals from M. fruticosa. Over 180 phytochemicals (87 flavonoids, 41 phenolic acids, 16 terpenoids, 8 sulfate derivatives, 7 iridoids, and others) are reported in Micromeria for the first time. M. fruticosa can be a promising source of functional ingredients and has use in the food, pharma and nutraceutical industries.