Herpes virus inhibitory substances from Hypericum connatum Lam., a plant used in southern Brazil to treat oral lesions

• Published in: Journal of ethnopharmacology Vol. 113; no. 3; pp. 517 - 520
• Main Authors: Fritz, Daniela, Venturi, Caroline Rita, Cargnin, Simone, Schripsema, Jan, Roehe, Paulo Michel, Montanha, Jarbas Alves, von Poser, Gilsane Lino
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 25.09.2007; Elsevier
• Subjects: amentoflavone; Animals; Antiherpetic activity; Antiviral Agents - pharmacology; antiviral properties; benzene; Biological and medical sciences; Brazil; Cercopithecus aethiops; chemical constituents of plants; flavonoids; Flavonoids - pharmacology; General pharmacology; guaijaverine; Guttiferae; herpes simplex; Herpes simplex viruses; hyperbrasilol; Hypericum; Hypericum - chemistry; Hypericum connatum; hyperoside; lesions (animal); Luteoforol; Medical sciences; medicinal plants; Medicine, Traditional; mouth; Mouth Diseases - drug therapy; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phloroglucinol - analogs & derivatives; Phloroglucinol - pharmacology; phloroglucinol derivative; Phytotherapy; plant extracts; Plant Extracts - pharmacology; Plants, Medicinal; Quercetin derivatives; Simplexvirus - drug effects; Vero Cells; Brazil; South America; America; Hypericum connatum; Antiherpetic activity; Luteoforol; Guttiferae; Quercetin derivatives; Herpes simplex viruses; Hypericum perforatum; Weed; Pharmacognosy; Herpesviridae; Flavonoid; Medicinal plant; Virus; Oral cavity; Polyphenol; Dicotyledones; Angiospermae; Plant origin; Quercetin; Phenols; Flavone derivatives; Antiviral; Spermatophyta
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.07.013

Hypericum connatum (Guttiferae) is used in southern Brazil in the treatment of lesions in the mouth, often related to acute herpetic gingivo-stomatitis. The chemical investigation of the plant revealed the presence of phloroglucinol derivatives and flavonoids. From the n-hexane extract of the aerial parts a phloroglucinol derivative, hyperbrasilol B, was isolated, while the methanolic extract afforded four flavonoids: amentoflavone, hyperoside, guaijaverine and luteoforol. The crude methanolic extract and fractions ( n-hexane, dichloromethane and methanol) as well as the isolated compounds were tested for antiviral activity against herpes simplex viruses (HSV). Among the tested samples, luteoforol was the most active inhibiting the cytopathic effect (CPE) and reducing the viral titer of HSV-1 DNA viral strains KOS and VR733 (ATCC).