Effects of low-power LED and therapeutic ultrasound in the tissue healing and inflammation in a tendinitis experimental model in rats

• Published in: Lasers in medical science Vol. 29; no. 1; pp. 301 - 311
• Main Authors: Moura Júnior, Manoel de Jesus, Arisawa, Emilia Ângela Loschiavo, Martin, Airton Abrahão, de Carvalho, Janderson Pereira, da Silva, José Mário Nunes, Silva, José Figueiredo, Silveira, Landulfo
• Format: Journal Article
• Language: English
• Published: London Springer London 2014; Springer Nature B.V
• Subjects: Animals; Collagen Type I - metabolism; Collagen Type III - metabolism; Dentistry; Disease Models, Animal; Fibroblasts - pathology; Inflammation - metabolism; Inflammation - pathology; Inflammation - therapy; Lasers; Light emitting diodes; Male; Medical treatment; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Photonics; Phototherapy - methods; Quantum Optics; Rats; Rats, Wistar; Spectrum analysis; Spectrum Analysis, Raman; Tendinopathy - metabolism; Tendinopathy - pathology; Tendinopathy - therapy; Tendons; Time Factors; Ultrasonic Therapy - methods; Wound Healing; FT-Raman spectroscopy; Induced tendinitis; Histomorphometry; LED and ultrasound therapies
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-013-1327-0

This work evaluated the anti-inflammatory response of low-power light-emitting diode (LED) and ultrasound (US) therapies and the quality and rapidness of tendon repair in an experimental model of tendinitis, employing histomorphometry and Raman spectroscopy. Tendinitis was induced by collagenase into the right tendon of 35 male Wistar rats with an average weight of 230 g. The animals were randomly separated into seven groups of five animals each: tendinitis without treatment—control (TD7 and TD14, where 1 and 2 indicated sacrifice on the 7th and 14th day, respectively), tendinitis submitted to US therapy (US7 and US14) and tendinitis submitted to LED therapy (LED7 and LED14). Contralateral tendons of the TD group at the 14th day were used as the healthy group (H). US treatment was applied in pulsed mode at 10 %, 1 MHz frequency, 0.5 W/cm 2 , 120 s. LED therapy parameters were 4 J/cm 2 , 120 s, daily dose at the same time and same point. Sacrifice was performed on the 7th or 14th day. Histomorphometric analysis showed lower number of fibroblasts on the 14th day of therapy for the US-treated group, compared to the TD and LED, indicating lower tissue inflammation. Raman showed that the LED group had an increase in the amount of collagen I and III from the 7th to the 14th day, which would indicate more organized fibers and a better quality of the healing, and US showed lower collagen I synthesis in the 14th day compared to H, indicating a lower tissue reorganization.