Polyamine transport systems in mammalian cells and tissues

Polyamine transport plays an important role in the homeostatic regulation of the polyamine levels. In animals, dietary polyamines are absorbed efficiently in the intestinal tract. In the colon, luminal bacterial derived polyamines are important contributors to cellular polyamine contents. Polyamine...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 720; p. 339
Main Authors Uemura, Takeshi, Gerner, Eugene W
Format Journal Article
LanguageEnglish
Published United States 2011
Subjects
Animals
Biological Assay - methods
Biological Transport
Cell Membrane - metabolism
CHO Cells
Chromatography, High Pressure Liquid
Colon - metabolism
Cricetinae
Cricetulus
HCT116 Cells
Humans
Intestine, Small - metabolism
Mammals - metabolism
Mice
Organ Specificity
Polyamines - metabolism
Putrescine - metabolism
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Summary:Polyamine transport plays an important role in the homeostatic regulation of the polyamine levels. In animals, dietary polyamines are absorbed efficiently in the intestinal tract. In the colon, luminal bacterial derived polyamines are important contributors to cellular polyamine contents. Polyamine transport involves unique uptake and export mechanisms. The amino acid transporter SLC3A2 acts as a polyamine exporter in colon cancer-derived cells. Polyamine uptake is mediated by caveolin-1 dependent -endocytosis. The K-RAS oncogene signals increased polyamine uptake and decreased polyamine export. Here, we describe the methods of polyamine transport analysis in the colon and the small intestine using -membrane vesicles, culture cells, and mouse models.
ISSN:1940-6029
DOI:10.1007/978-1-61779-034-8_21