Characterization of duplicated heme oxygenase-1 genes and their responses to hypoxic stress in blunt snout bream (Megalobrama amblycephala)
The heme oxygenase (HO)-1 is a cytoprotective enzyme that can be involved in cytoprotection against hypoxia stress. In this study, we cloned duplicated HO-1a and HO-1b cDNAs in hypoxia-sensitive blunt snout bream ( Megalobrama amblycephala ). HO-1a and HO-1b encode peptides with 272 amino acids and...
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Published in | Fish physiology and biochemistry Vol. 43; no. 2; pp. 641 - 651 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.04.2017
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The heme oxygenase (HO)-1 is a cytoprotective enzyme that can be involved in cytoprotection against hypoxia stress. In this study, we cloned duplicated
HO-1a
and
HO-1b
cDNAs in hypoxia-sensitive blunt snout bream (
Megalobrama amblycephala
).
HO-1a
and
HO-1b
encode peptides with 272 amino acids and 246 amino acids, respectively, and they share a low sequence identity of 55%.
HO-1a
and
HO-1b
mRNAs were maternally deposited in the zygote, and the mRNAs decreased to the lowest levels at 8 hpf. Both mRNAs were significantly (
p
< 0.01) expressed from 12 hpf and fluctuated but maintained a high level after 16 hpf. Using in situ hybridization,
HO-1a
and
HO-1b
mRNAs were ubiquitously expressed in embryos at 12 hpf. At 24 and 36 hpf,
HO-1b
transcripts were detected in the mid- and hindbrain, respectively, whereas
HO-1a
was mainly transcribed in the eyes and endoderm at 24 hpf and in the brain at 36 hpf. In adult fish,
HO-1a
was abundantly expressed in the heart, liver, gill, kidney, spleen, and brain, while
HO-1b
mRNA was detected mainly in the kidney. After exposure to hypoxic stress, both
HO-1a
and
HO-1b
mRNAs were upregulated significantly in the gill and liver but downregulated significantly in the brain (
p
< 0.01). These findings suggest that duplicated
HO
genes have evolved divergently and yet play overlapping biological roles in regulating the response to hypoxia in
M. amblycephala
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0920-1742 1573-5168 |
DOI: | 10.1007/s10695-016-0318-z |