Evaluation of cytotoxicity of hematite nanoparticles in bacteria and human cell lines

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 157; pp. 101 - 109
• Main Authors: Rajendran, Kumar, Sen, Shampa, G., Suja, Senthil, S. Lakshmana, Kumar, T. Vinoth
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2017
• Subjects: A549 Cells; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bacillus cereus - drug effects; Bacteria; Cell Line; Cell Survival - drug effects; Cercopithecus aethiops; Cytotoxic; Erythrocytes - drug effects; Ferric Compounds - chemistry; Ferric Compounds - toxicity; Hematite nanoparticle; Hemolysis - drug effects; Hemolytic; Humans; MCF-7 Cells; Metal Nanoparticles - toxicity; Optimization; Particle Size; Vero Cells; Bacteria; Hemolytic; Cytotoxic; Hematite nanoparticle; Optimization
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2017.05.052

[Display omitted] •Rapid biosynthesis of hematite nanoparticle was achieved by media optimization.•Chemical composition of nanoparticles primarily determines the antibacterial activity.•Hematite nanoparticles caused significant cytotoxicity in cancer cells.•Hematite nanoparticles were found to be biocompatible as they didn’t cause hemolysis of RBC. Metal nanoparticles have a great impact and even change the composition of soil microbial communities. This poses the risk of their accumulation in the ecosystem, which may call on health hazard. Statistical techniques such as Plackett-Burman design, Response Surface Methodology were used for optimizing medium constituents for Bacillus cereus SVK1 and other critical variables responsible for the production of biomolecules and biosynthesis of hematite nanoparticles. The effect of hematite nanoparticles on the growth of soil bacteria were tested by agar-well diffusion method and dynamic growth curve techniques. Bacterial growth inhibition was not observed with hematite nanoparticle concentration of up to 25mg/mL. In addition, hematite nanoparticles enhanced the growth of the soil bacteria. The results show that hematite nanoparticles were non-toxic to soil bacteria indicating their scope for wide range of applications. The effective cytotoxic concentration (CTC50) of hematite nanoparticles against MCF-7, A549, Hep3B and Vero cell lines was found to be 207.58, 224.69, 193.26 and 2530μg/mL respectively. Hematite nanoparticles didn’t cause lysis of red blood cells.