Orthogonal array design for optimizing extraction efficiency of active constituents from Jakyak-Gamcho Decoction, the complex formula of herbal medicines, Paeoniae Radix and Glycyrrhizae Radix

• Published in: Journal of ethnopharmacology Vol. 113; no. 2; pp. 306 - 311
• Main Authors: Guo, Lianyu, Cho, So Yean, Kang, Sam Sik, Lee, Seung-Ho, Baek, Hum-Young, Kim, Yeong Shik
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 05.09.2007; Elsevier
• Subjects: Alkaline Phosphatase - genetics; Alkaline Phosphatase - metabolism; Animals; anti-inflammatory activity; Benzoates - chemistry; Benzoates - isolation & purification; bioassays; Biological and medical sciences; Bridged-Ring Compounds - chemistry; Bridged-Ring Compounds - isolation & purification; Cell Line; Cell Survival - drug effects; Chalcone - analogs & derivatives; Chalcone - chemistry; Chalcone - isolation & purification; chemical constituents of plants; Chemical Fractionation - methods; Chromatography, High Pressure Liquid; Ethanol - chemistry; ethnobotany; extraction; Flavanones - chemistry; Flavanones - isolation & purification; General pharmacology; Glucosides - chemistry; Glucosides - isolation & purification; Glycyrrhiza - chemistry; Glycyrrhiza uralensis; Glycyrrhiza uralensis Fisch; Glycyrrhizic Acid - chemistry; Glycyrrhizic Acid - isolation & purification; Herbal Medicine; herbal medicines; inflammation; Jakyak-Gamcho Decoction; Lipopolysaccharides - pharmacology; Macrophages - cytology; Macrophages - drug effects; Macrophages - metabolism; Medical sciences; medicinal plants; medicinal properties; mice; Monoterpenes; NF-kappa B - genetics; NF-kappa B - metabolism; Nitric Oxide - metabolism; optimization; Optimization of extraction; Oriental traditional medicine; Orthogonal array design; Paeonia - chemistry; Paeonia lactiflora; Paeonia lactiflora PALL; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; plant extracts; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Research Design; roots; Solubility; Technology, Pharmaceutical - methods; Water - chemistry; South Korea; Asia; China; PBS; NO; Orthogonal array design; TPCK; JGD; Optimization of extraction; SEAP; Paeonia lactiflora PALL; AMT; LPS; DMEM; NF-κB; NPT; FBS; HEPES; Jakyak-Gamcho Decoction; CCK-8; 4-MUP; HPLC; Glycyrrhiza uralensis Fisch; Root; Pharmacognosy; Ranunculaceae; Decoction; Paeonia lactiflora; Optimization; Medicinal plant; Folk medicine; Efficiency; Dicotyledones; Angiospermae; Plant origin; Flower crop; Spermatophyta; Extraction; Paeoniaceae; Below ground plant part
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.06.013

A complex formula composed of Paeonia lactiflora PALL. and Glycyrrhiza uralensis Fisch., which is called as Jakyak-Gamcho Decoction (JGD), has been used for a pain-relieving function and muscle spasms due to blood deficiency in the traditional medicine. In this study, the anti-inflammatory activity of JGD was evaluated based on the quantitative determinations and the relative proportions of six major constituents in the decoction mixture extracted by orthogonal array methods. Our results suggest that the three parameters are all crucial factors. The optimized conditions for extraction were therefore established [solvent (water); pH value (4); extraction number (4)]. We also optimized the extraction conditions related to anti-inflammatory activity [solvent (70% EtOH); pH value (6); extraction number (4)]. So, we found that the bioactivity was responsible for mixed components but not individual one. It was proportionally associated with the amounts of some components in the extracts of herbal medicines. When the proportion of the active components was similar to each other, they had the similar functions. Furthermore, the results could establish a model system for the quality assurance of herbal preparations, and provided a new paradigm of active components-pharmacodynamics, which is used for illustrating the connections between the bioactivities and the proportion of active constituents in the extracts of herbal medicines.