Extraction, selenylation modification and antitumor activity of the glucan from Castanea mollissima Blume

• Published in: Glycoconjugate journal Vol. 34; no. 2; pp. 207 - 217
• Main Authors: Li, Hongyan, Wang, Yingxing, Wang, Chao, Zhang, Shaohan, Li, Shenghui, Zhou, Guoqiang, Wang, Shuxiang, Zhang, Jinchao
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2017; Springer Nature B.V
• Subjects: Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis; Apoptosis - drug effects; Biochemistry; Biomedical and Life Sciences; Caspase 3 - metabolism; Castanea mollissima; Fagaceae - chemistry; Flowers - chemistry; Glucans - chemistry; Glucans - isolation & purification; Glucans - pharmacology; HeLa Cells; Humans; Life Sciences; MCF-7 Cells; Molecular biology; Neoplasm Proteins - metabolism; Neoplasms - drug therapy; Neoplasms - metabolism; Original Article; Pathology; Selenium - chemistry; Selenium - pharmacology; Studies; Tumors; Glucan; Selenylation modification; Antitumor activity; Blume; Apoptosis; Castanea mollissima Blume
• ISSN: 0282-0080; 1573-4986
• DOI: 10.1007/s10719-016-9753-4

The present study aimed to characterize the glucan from C. mollissima Blume fruits and its selenium derivative, then investigate their antitumor activity in vitro . A glucan, designated as CPA, was firstly isolated from the fruits of C. mollissima Blume. Structure analysis indicated that CPA was a linear 1,6-α-D-glucan with the average molecular weight about 2.0 × 10 3  kDa. The selenylation modification derivative of CPA (sCPA), exhibited a stronger antiproliferative effect on tumor cells than CPA in vitro . CPA and sCPA could induce HeLa cells apoptosis and decrease mitochondrial membrane potential. sCPA could also arrest HeLa cells in S phase, promote reactive oxygen species generation and activate caspase-3 activity in HeLa cells. These results manifest that CPA and sCPA inhibit the proliferation of HeLa cells via different mechanisms, which is meaningful for their potential use as antitumor drugs.