Evaluation of antimicrobial, antioxidant and wound-healing potentials of Holoptelea integrifolia

• Published in: Journal of ethnopharmacology Vol. 115; no. 2; pp. 249 - 256
• Main Authors: Srinivas Reddy, Boreddy, Kiran Kumar Reddy, R., Naidu, V.G.M., Madhusudhana, K., Agwane, Sachin B., Ramakrishna, Sistla, Diwan, Prakash V.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 17.01.2008; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Animals; Anti-Infective Agents - administration & dosage; Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; antimicrobial properties; antioxidant activity; antioxidants; Antioxidants - administration & dosage; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bacteria - drug effects; bark; Biological and medical sciences; collagen; Disease Models, Animal; DPPH; Free Radicals - metabolism; Fungi - drug effects; General pharmacology; histopathology; Holoptelea integrifolia; Holoptelea integrifolia (Roxb.) (Urticaceae); Inhibitory Concentration 50; leaves; Male; Medical sciences; medicinal plants; medicinal properties; Microbial Sensitivity Tests; Mimimum microbicidal concentration; Minimum inhibition concentration; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Bark; plant extracts; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Leaves; Rats; Rats, Wistar; reactive oxygen species; Reactive Oxygen Species - metabolism; Tensile strength; tissue repair; Total phenolic content; Urticaceae; Urticaceae - chemistry; Wound Healing - drug effects; Total phenolic content; Holoptelea integrifolia (Roxb.) (Urticaceae); Minimum inhibition concentration; DPPH; Mimimum microbicidal concentration; Tensile strength; Evaluation; Pharmacognosy; Antioxidant; Wound; Antimicrobial agent; Medicinal plant; Urticaceae; Healing agent; Dicotyledones; Content; Angiospermae; Plant origin; Spermatophyta
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.09.031

The methanolic extracts of Holoptelea integrifolia (Roxb.) (Urticaceae) leaves (MLE) and stem bark (MSBE) were studied for the wound-healing potential. Since wound healing is severely hampered by microbial infection and reactive oxygen species (ROS), this study was undertaken to evaluate antimicrobial and antioxidant activity apart from wound-healing activity. The antimicrobial property of the Holoptelea was studied against the six bacterial and five fungal strains using the agar well diffusion method and minimum microbicidal concentration and minimum inhibitory concentration were determined for each strain, in which methanolic extract of stem bark (MSBE) has shown bigger zone of inhibition (11.3–20.4 mm) than methanolic extract of leaves (MLE) (9.6–14.9 mm). The anti-oxidant activity was evaluated by DPPH free radical scavenging activity using HPLC method. The IC 50 values obtained for MSBE (TPC: 78.53 ± 1.26 mg/g) and MLE (TPC: 57.71 ± 1.45 mg/g) were 37.66 ± 0.48 and 50.36 ± 0.59 μg/well, respectively. In excision wound model, more than 90% wound healing was recorded in treated groups by 14 days of post surgery, where as only 62.99% was observed in the control group. In incision model, higher breaking strengths and higher hydroxyproline content in treated groups suggested higher collagen re-deposition than the control group. Finally, histopathology studies conformed wound-healing activity of Holoptelea integrifolia.