Characterization of a novel dsRNA mycovirus of Trichoderma atroviride NFCF028

• Published in: Archives of virology Vol. 162; no. 4; pp. 1073 - 1077
• Main Authors: Lee, Song Hee, Yun, Suk-Hyun, Chun, Jeesun, Kim, Dae-Hyuk
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.04.2017; Springer Nature B.V
• Subjects: Base Sequence; Biomedical and Life Sciences; Biomedicine; Brief Report; Cloning; Fungal Viruses - chemistry; Fungal Viruses - classification; Fungal Viruses - genetics; Fungal Viruses - isolation & purification; Genome, Viral; Genomes; Infectious Diseases; Medical Microbiology; Molecular biology; Molecular Sequence Data; Mushrooms; Nucleic Acid Conformation; Open Reading Frames; Phylogenetics; Phylogeny; Proteins; RNA polymerase; RNA, Double-Stranded - chemistry; RNA, Double-Stranded - genetics; RNA, Viral - chemistry; RNA, Viral - genetics; Trichoderma - virology; Trichoderma atroviride; Viral Proteins - genetics; Virology; Viruses; Shiitake; Mycovirus; Trichoderma atroviride; Lentinula edodes
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-016-3214-z

Molecular characterization of the most common dsRNA element from Trichoderma atroviride indicated that it comprised 8,566 bp and encoded two large open reading frames (ORF1 and 2). The two ORFs were found to overlap by 46 bp with a typical (−1) slippery heptanucelotide sequence. The deduced protein sequences of ORF1 and ORF2 showed significant similarities to those of known mycoviral structural proteins and RNA-dependent RNA polymerases, respectively. Phylogenetic analysis indicated that this dsRNA is a member of a distinct species related to a group of unclassified mycoviruses; therefore, it was named Trichoderma atroviride mycovirus 1 (TaMV1).