Toward Fully Automated High-Resolution Electron Tomography

Electron tomography is a powerful tool in elucidating the three-dimensional architecture of large biological complexes and subcellular organelles. Its use can be expanded through the simplification of the tomographic procedure by automation of its tasks. In this paper, we describe our EMACT/EMCAT sy...

Full description

Saved in:
Bibliographic Details
Published inJournal of structural biology Vol. 116; no. 1; pp. 181 - 189
Main Authors Fung, Jennifer C., Liu, Weiping, de Ruijter, W.J., Chen, Hans, Abbey, Craig K., Sedat, John W., Agard, David A.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1996
Subjects
Animals
Automation
Centrosome - ultrastructure
Computer Graphics
Computer Simulation
Microscopy, Electron
Models, Structural
Organelles - ultrastructure
Software
Online AccessGet full text

Cover

More Information
Summary:Electron tomography is a powerful tool in elucidating the three-dimensional architecture of large biological complexes and subcellular organelles. Its use can be expanded through the simplification of the tomographic procedure by automation of its tasks. In this paper, we describe our EMACT/EMCAT system, which automates both tomographic data collection and reconstruction.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1047-8477
1095-8657
DOI:10.1006/jsbi.1996.0029