Biocompatibility of a chitosan-based injectable thermosensitive hydrogel and its effects on dog periodontal tissue regeneration

• Published in: Carbohydrate polymers Vol. 82; no. 4; pp. 1153 - 1160
• Main Authors: Ji, Qiu Xia, Deng, Jing, Xing, Xiao Ming, Yuan, Chang Qing, Yu, Xin Bo, Xu, Quan Chen, Yue, Jin
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 11.11.2010; Elsevier
• Subjects: Applied sciences; Biological and medical sciences; Chitosan; Exact sciences and technology; General pharmacology; Local drug delivery system; Medical sciences; Natural polymers; Periodontal disease; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Physicochemistry of polymers; Starch and polysaccharides; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Thermosensitive hydrogel; Tissue regeneration; Tissue-engineering scaffold; Periodontal disease; Tissue-engineering scaffold; Chitosan; Tissue regeneration; Local drug delivery system; Thermosensitive hydrogel; Biological properties; Fibroblast growth factor; Injectable form; Parodontium; Cytotoxicity; Drug carrier; Glycerophosphate; Biocompatibility; Biomaterial; Framework; Tissue engineering; Control release polymer; Gelation; Inflammation; Experimental study; In vitro; In vivo; Regeneration; Chitosan derivatives; Animal; Oside polymer; Kinetics; Hydrogel; Aqueous solution; Quaternary ammonium polymer
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2010.06.045

We evaluated the biocompatibility of an injectable chitosan-based thermosensitive hydrogel, which was prepared from chitosan and quaternized chitosan (HTCC) containing α,β-GP (CS-HTCC/GP). The ability of the CS-HTCC/GP thermosensitive hydrogel to partake in periodontal tissue regeneration was investigated. The CS-HTCC/GP thermosensitive hydrogel was found to have no toxic effects and could promote alkaline phosphatase (ALP) activity in human periodontal ligament cells (HPDLCs) in vitro. An extract of the CS-HTCC/GP thermosensitive hydrogel had no obvious influence on the ultrastructure of HPDLCs. The CS-HTCC/GP solution was injected into the rumps of rats resulting in a non-specific inflammation reaction. CS-HTCC/GP thermosensitive hydrogel loading with basic fibroblast growth factor (CS-HTCC/GP-bFGF) more effectively enhanced new periodontal support tissues in dogs. All results indicated that the CS-HTCC/GP thermosensitive hydrogel exhibited excellent biocompatibility and had potential as an injectable local drug delivery vehicle and tissue-engineering scaffold for periodontal disease therapy.