Multiplexed suspension array platform for high-throughput protein assays

A multiplexed suspension array platform, based on SU8 disks patterned with machine-readable binary identification codes is presented. Multiple probe molecules, each attached to individual disks with different unique codes, provide multiplexed detection of targets in a small sample volume. The experi...

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Bibliographic Details
Published inBiomedical microdevices Vol. 14; no. 4; pp. 651 - 657
Main Authors Birtwell, S. W., Broder, G. R., Roach, P. L., Morgan, H.
Format Journal Article
LanguageEnglish
Published Boston Springer US 01.08.2012
Springer Nature B.V
Subjects
Biological and Medical Physics
Biomedical Engineering and Bioengineering
Biomedical materials
Biophysics
Biotechnology
Cytokines
Drug Discovery
Engineering
Engineering Fluid Dynamics
Equipment Design
High-Throughput Screening Assays - instrumentation
Humans
Immunoassay
Molecular biology
Nanotechnology
Protein Array Analysis - instrumentation
Proteins
Suspensions
Immunoassay
Bead-based
Microarray
Encoded microparticles
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Summary:A multiplexed suspension array platform, based on SU8 disks patterned with machine-readable binary identification codes is presented. Multiple probe molecules, each attached to individual disks with different unique codes, provide multiplexed detection of targets in a small sample volume. The experimental system consists of a microfluidic chamber for arraying the particles in a manner suitable for high throughput imaging using a simple fluorescent microscope, together with custom software for automated code readout and analysis of assay response. The platform is demonstrated with a multiplexed antibody assay targeting 3 different human inflammatory cytokines. The suitability of the platform for other bio-analytical applications is discussed.
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ISSN:1387-2176
1572-8781
DOI:10.1007/s10544-012-9641-z