Cloning and Characterization of a Novel Class I Cytokine Receptor

• Published in: Biochemical and biophysical research communications Vol. 246; no. 1; pp. 82 - 90
• Main Authors: Sprecher, Cindy A., Grant, Francis J., Baumgartner, James W., Presnell, Scott R., Schrader, Sara K., Yamagiwa, Tina, Whitmore, Theodore E., O'Hara, Patrick J., Foster, Donald F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08.05.1998
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Brain - metabolism; Chromosome Mapping; Cloning, Molecular; Conserved Sequence; DNA Primers - genetics; DNA, Complementary - genetics; Humans; Hybrid Cells; Infant; Ligands; Mice; Molecular Sequence Data; Receptors, Cytokine - classification; Receptors, Cytokine - genetics; Receptors, Cytokine - metabolism; Receptors, Interleukin; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Signal Transduction; Tissue Distribution
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1998.8576

The human gp130 cDNA sequence was used as a query to search an expressed sequence tag database (dbEST) to identify cDNA sequences with similarity to the cytokine class I receptor family. A novel class I cytokine receptor was identified in a human infant brain cDNA library and was named WSX-1. Full-length cDNA sequences for human and murine WSX-1 were isolated and characterized. The WSX-1 cDNA encodes a 636 amino acid transmembrane protein with an extracellular domain of 482 amino acids and a cytoplasmic domain of 96 amino acids. The structure of the WSX-1 protein most closely resembles that of gp130. Northern blot analysis indicates high levels of expression in thymus, spleen, lymph node, and peripheral blood leukocytes, suggesting a role for WSX-1 in modulation of the immune response.