Bioactive-glass ceramic with two crystalline phases (BioS-2P) for bone tissue engineering

• Published in: Biomedical materials (Bristol) Vol. 12; no. 4; p. 045018
• Main Authors: Prado Ferraz, Emanuela, Pereira Freitas, Gileade, Camuri Crovace, Murilo, Peitl, Oscar, Dutra Zanotto, Edgar, de Oliveira, Paulo Tambasco, Mateus Beloti, Marcio, Luiz Rosa, Adalberto
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 09.08.2017
• Subjects: Alkaline Phosphatase - metabolism; Animals; bioactive glass-ceramic; Biosilicate; bone; Bone and Bones - metabolism; Bone Regeneration; Cell Line; Cells, Cultured; Ceramics - chemistry; Core Binding Factor Alpha 1 Subunit - metabolism; Gene Expression Regulation; Glass - chemistry; Integrin-Binding Sialoprotein - metabolism; Materials Testing; mesenchymal stem cell; Mesenchymal Stromal Cells - cytology; osteoblast; Osteogenesis; Polystyrenes - chemistry; Rats; scaffold; Tissue Engineering - methods; Tissue Scaffolds; X-Ray Diffraction
• ISSN: 1748-6041; 1748-605X; 1748-605X
• DOI: 10.1088/1748-605X/aa768e

We aimed to evaluate the in vitro osteogenic and osteoinductive potentials of BioS-2P and its ability to promote in vivo bone repair. To investigate osteogenic potential, UMR-106 osteoblastic cells were cultured on BioS-2P and Bioglass 45S5 discs in osteogenic medium. The osteoinductive potential was evaluated using mesenchymal stem cells (MSCs) cultured on BioS-2P, Bioglass 45S5 and polystyrene in non-osteogenic medium. Rat bone calvarial defects were implanted with BioS-2P scaffolds alone or seeded with MSCs. UMR-106 proliferation was similar for both materials, while alkaline phosphatase (ALP) activity and mineralization were higher for BioS-2P. Bone sialoprotein (BSP), RUNX2 and osteopontin (OPN) gene expression and BSP, OPN, ALP and RUNX2 protein expression were higher on BioS-2P. For MSCs, ALP activity was higher on Bioglass 45S5 than on BioS-2P and was lower on polystyrene. All genes were highly expressed on bioactive glasses compared to polystyrene. BioS-2P scaffolds promoted in vivo bone formation without differences in the morphometric parameters at 4, 8 and 12 weeks. After 8 weeks, the combination of BioS-2P with MSCs did not increase the quantity of new bone compared to the BioS-2P alone. To stimulate osteoblast activity, drive MSC differentiation and promote bone formation, BioS-2P is a good choice as a scaffold for bone tissue engineering.