Sulfated modification, characterization and structure–antioxidant relationships of Artemisia sphaerocephala polysaccharides

• Published in: Carbohydrate polymers Vol. 81; no. 4; pp. 897 - 905
• Main Authors: Wang, Junlong, Guo, Hongyun, Zhang, Ji, Wang, Xiaofang, Zhao, Baotang, Yao, Jian, Wang, Yunpu
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 23.07.2010; Elsevier
• Subjects: Antioxidant activities; Applied sciences; Artemisia sphaerocephala; Biological and medical sciences; Chain conformation; Exact sciences and technology; General and cellular metabolism. Vitamins; Medical sciences; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharides; Starch and polysaccharides; Sulfation; Chain conformation; Polysaccharides; Antioxidant activities; Artemisia sphaerocephala; Sulfation; Metal ion; Compositae; Medicinal plant; Radical trapping; Chinese medicine; Binding capacity; Scavenging efficiency; Structure activity relation; Dicotyledones; Angiospermae; Organic sulfate; Hydroxyl radicals; DPPH; Regioselectivity; Iron II; Antioxidant; Experimental study; Biological activity; Sulfatation; Chemical modification; Preparation; Plant origin; Oside polymer; Spermatophyta; Superoxide radicals
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2010.04.002

Sulfated polysaccharides exerted potent biological property which was relative to degree of sulfation (DS), M w, substitution position and chain conformation. In the present study, sulfated derivatives of Artemisia sphaerocephala polysaccharide (ASP) with different DS were synthesized by chlorosulfuric acid/pyridine method. FT-IR and 13C NMR analysis indicated that C-6 substitution was predominant in sulfated ASP (SASP) compared with other positions at C-2. In the sulfation reaction, a sharp decrease in M w was observed in SASP. The d f values from 1.96 to 2.77 indicated that the –SO 3H groups leading to the relatively expanded conformation of SASP. Antioxidant assays showed that SASP had better antioxidant activities. The data obtained in vitro models indicated that high DS and moderate M w showed the best antioxidant capacities.