Conservation of the 5' end of the medRNA in New World species of Leishmania

The effectiveness of antisense oligonucleotides targeted against the spliced leader (SL) in trypanosomes has been documented by Verspieren at al. To evaluate a similar approach for leishmaniasis antisense therapy, we used a 12 base primer (Primer A) complementary to a conserved region of the SL to s...

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Bibliographic Details
Published inNucleic acids research Vol. 17; no. 19; p. 7999
Main Authors SCHOLLER, J. K, MCARDLE, S, REED, S, KANEMOTO, R
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 11.10.1989
Subjects
Animals
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genes. Genome
Leishmania
Leishmania - genetics
Leishmania braziliensis
Leishmania donovani
Leishmania mexicana
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Protein Sorting Signals
RNA Precursors - genetics
RNA, Messenger - genetics
Sequence Homology, Nucleic Acid
Species Specificity
Conserved sequence
5'-End
Complementary DNA
Nucleotide sequence
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Summary:The effectiveness of antisense oligonucleotides targeted against the spliced leader (SL) in trypanosomes has been documented by Verspieren at al. To evaluate a similar approach for leishmaniasis antisense therapy, we used a 12 base primer (Primer A) complementary to a conserved region of the SL to sequence the 5' portion of the SL from the medRNA and the mature mRNAs of several leishmanial species. The first 15 nucleotides of the spliced leader was identical in the New World species of L. braziliensis quyanensis (Lbg), L. mexicana amazonensis (Lma), L. donovani chagasi (Ldc) and L. enriettii (Le).
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.19.7999