The B-L (Ia-like) antigens of the chicken. lymphocyte plasma membrane distribution and tissue localization

• Published in: Developmental and comparative immunology Vol. 8; no. 3; pp. 673 - 682
• Main Authors: Hála, K., Wick, G., Boyd, R.L., Wolf, H., Böck, G., Ewert, D.L.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.01.1984; Elsevier Science
• Subjects: Animals; ANTIGENE; ANTIGENOS; ANTIGENS; B-Lymphocytes - immunology; Biological and medical sciences; Brain - immunology; Cell Membrane - immunology; CELL STRUCTURE; CHICKENS; Chickens - immunology; ESTRUCTURA CELULAR; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Histocompatibility Antigens Class II - isolation & purification; LINFOCITOS; LYMPHOCYTE; LYMPHOCYTES; Lymphocytes - immunology; Molecular immunology; POLLO; POULET; STRUCTURE CELLULAIRE; T-Lymphocytes - immunology; TEJIDO (ANATOMIA); TISSU; Tissue Distribution; TISSUES; Antigen; Vertebrata; Major histocompatibility system; Plasma membrane; Chicken; Indirect immunofluorescence; Aves; Localization; Lymphocyte
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/0145-305X(84)90099-5

Specific antisera reacting with B-L (Ia-like) antigens were prepared by reciprocal immunization of animals from the congeneic lines CB and CC. The resulting antisera were tested either in direct or indirect immunofluorescence tests and stained 10–16% of peripheral blood cells (PBL). Of the B-L + cells, 90% were B cells and 8% were T cells. After in vitro stimulation of PBL with Con A, 58% were B-L + and 91% of these were T cells. B and T cells were defined by means of rabbit antisera raised against bursa and thymus cells made specific by absorption with the relevant tissues. Antigens determined by anti-B-L antisera, rabbit anti-bursa (ABS) and rabbit anti-thymus (ATS) sera showed an independent distribution on the membrane of PBL. The tissue distribution of B-L + cells, defined by means of allo-antisera and monoclonal antibodies, was studied by direct and indirect immunofluorescence on sections of skin, liver, kidney and brain. In all organs, in addition to B cells and a small number of, presumably activated, T cells, macrophages and dendritic cells were positive. Notably, glia cells in the brain were also shown to express B-L antigen.