Antinociceptive activity of Calotropis procera latex in mice

• Published in: Journal of ethnopharmacology Vol. 99; no. 1; pp. 125 - 129
• Main Authors: Soares, Paula Matias, Lima, Silvane R., Matos, Samara G., Andrade, Marcelo M., Patrocínio, Manoel C.A., de Freitas, Cleverson D.T., Ramos, Márcio V., Criddle, David N., Cardi, Bruno A., Carvalho, Krishnamurti M., Assreuy, Ana Maria S., Vasconcelos, Silvânia M.M.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 13.05.2005; Elsevier
• Subjects: Acetic Acid; analgesic effect; Analgesics - pharmacology; Animals; Antinociceptive activity; Biological and medical sciences; Calotropis - chemistry; Calotropis procera; Dose-Response Relationship, Drug; Formaldehyde; General pharmacology; Hot Temperature; latex; Latex - pharmacology; Latex proteins; Male; Medical sciences; medicinal plants; Mice; Naloxone - pharmacology; Narcotic Antagonists - pharmacology; Pain Measurement - drug effects; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; plant proteins; Plant Proteins - chemistry; Plant Proteins - pharmacology; Reaction Time - drug effects; Antinociceptive activity; Latex proteins; Calotropis procera; Pharmacognosy; Asclepiadaceae; Rodentia; Latex; Medicinal plant; Vertebrata; Mammalia; Analgesic; Mouse; Dicotyledones; Animal; Angiospermae; Plant origin; Spermatophyta
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2005.02.010

This work evaluated the antinociceptive effect of proteins from the Calotropis procera (Asclepiadaceae) latex using three different experimental models of nociception in mice. The latex protein fraction administered intraperitoneally in male mice at the doses of 12.5, 25 and 50 mg/kg showed the antinociceptive effect in a dose dependent manner compared to the respective controls in all assays. Inhibitions of the acetic acid-induced abdominal constrictions were observed at the doses of 12.5 (67.9%), 25 (85%) and 50 (99.5%) mg/kg compared to controls. Latex protein at the doses of 25 (39.8%; 42%) and 50 mg/kg (66.6%; 99.3%) reduced the nociception produced by formalin in the 1st and 2nd phases, respectively, and this effect was not reversed by pretreatment with naloxone (1 mg/kg). In the hot plate test, an increase of the reaction time was observed only at 60 min after the treatment with latex at the doses of 25 (79.5%) and 50 (76.9%) mg/kg, compared to controls and naloxone was ineffective to reverse the effect. It was concluded that the protein fraction derived from the whole latex of Calotropis procera possesses antinociceptive activity, which is independent of the opioid system.