Characterization of Ciprofloxacin-Resistant and Ciprofloxacin-Susceptible Uropathogenic Escherichia coli Obtained from Patients with Gynecological Cancer

• Published in: Current microbiology Vol. 73; no. 5; pp. 624 - 632
• Main Authors: Capett, Muniqui S., Vollú-Silva, Patricia, Melchiades, Vanessa A., Bokehi, Luciana C., Araújo, Fernanda M., Martins, Ianick Souto, Neves, Felipe P. G., Gonzalez, Alice G. M., Oswald, Eric, de Paula, Geraldo R., Teixeira, Lenise A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2016; Springer Nature B.V
• Subjects: Anti-Bacterial Agents - pharmacology; Biomedical and Life Sciences; Biotechnology; Cancer; Ciprofloxacin - pharmacology; Drug resistance; Drug Resistance, Bacterial; E coli; Escherichia coli; Escherichia coli Infections - microbiology; Escherichia coli Proteins - genetics; Female; Genital Neoplasms, Female - complications; Humans; Life Sciences; Microbial Sensitivity Tests; Microbiology; Phylogenetics; Phylogeny; Urinary tract infections; Urinary Tract Infections - etiology; Urinary Tract Infections - microbiology; Uropathogenic Escherichia coli - classification; Uropathogenic Escherichia coli - drug effects; Uropathogenic Escherichia coli - genetics; Uropathogenic Escherichia coli - isolation & purification; Gynecological Cancer; Susceptible Isolate; Virulence Factor Gene; Fluoroquinolone Resistance; Phylogenetic Group
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-016-1104-y

The objective of this work was to assess the genetic characteristics of uropathogenic Escherichia coli, ciprofloxacin resistance or susceptibility, obtained from patients with gynecological cancer and urinary tract infection (UTI). Seventy-seven E. coli ciprofloxacin-resistant isolates and 38 ciprofloxacin-susceptible were analyzed by polymerase chain reaction (PCR) to determine the phylogenetic groups, virulence factors as iuc C, fyu A, hly C, cnf 1 genes, and pks pathogenicity island. The presence of genes related to ciprofloxacin resistance such as qnr A, qnr B, qnr S, aac ( 6 ′)- Ib - cr , and qep A, and the sequencing of DNA gyrase genes and topoisomerase IV were determined. The genetic profile of the isolates was determined by pulsed-field gel electrophoresis (PFGE). Statistical analysis was performed using Fisher’s exact test and Chi-square test. Phylogenetic group B2 was the most prevalent although a great genetic diversity was observed by PFGE. Only genes associated to siderophores were found in ciprofloxacin-resistant isolates; however, in ciprofloxacin-susceptible isolates, genes related to siderophores and toxin, were detected. Additionally qnrB was detected in both populations, ciprofloxacin resistant and susceptible. DNA mutations in gyr A were Ser-83-Leu and Asp-87-Asn and in par C were Ser-80-Ile and Glu-84-Val, Glu-84-Lys. In conclusion, it was observed a high prevalence of qnr B in the population studied; in addition, it was the first time the pks island was observed only in ciprofloxacin-susceptible isolates.