Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. The pH-shifting method, as an ecofriendly approach, is a promising alternative to the desolvation method, yet systematic comparison of their properties is s...

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Published inJournal of dairy science Vol. 108; no. 1; pp. 282 - 297
Main Authors Zhong, Weigang, Xu, Lei, Wang, Qi, Shen, Xue
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.2025
Elsevier
Subjects
Animals
bovine serum albumin
Cattle
Ethanol
ethanol desolvation
galangin
Hydrogen-Ion Concentration
Inflammation
Macrophages
Mice
Nanoparticles
pH-shifting
Reactive Oxygen Species - metabolism
Serum Albumin, Bovine - chemistry
ethanol desolvation
pH-shifting
nanoparticles
galangin
bovine serum albumin
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Summary:The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. The pH-shifting method, as an ecofriendly approach, is a promising alternative to the desolvation method, yet systematic comparison of their properties is still lacking. In this study, BSA-galangin nanoparticles (BSA-GA NP) were designed for alleviating reactive oxygen species (ROS)–mediated macrophage inflammation by the 2 separate methods. Compared with the desolvation method, BSA exhibited a higher loading capacity for GA under the pH-shifting method, which was attributed to the exposure of the binding site leading to enhanced affinity for GA and a more compact particle structure. Further analyses evidenced that the electron arrangement and crystal structure of GA changed with different methods. The content of the random coil of BSA was elevated after the pH-shifting method. Additionally, the smaller size rendered the pH-shifting treated BSA-GA NP easier to be taken up by macrophages, and the enhanced specific surface area conferred excellent ROS scavenging and anti-inflammatory performances. This study may provide new insights into the choice of loading methods. [Display omitted]
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ISSN:0022-0302
1525-3198
1525-3198
DOI:10.3168/jds.2024-25495