Molecular Cloning and Tissue Expression of Human Mitochondrial 3-Hydroxy-3-Methylglutaryl-CoA Synthase

Mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase is a constituent of the HMG-CoA pathway responsible for ketone body synthesis and one of its main regulatory points. We report the isolation and characterization of a 2058-bp cDNA from human liver. This cDNA encodes a polypeptide...

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Bibliographic Details
Published inArchives of biochemistry and biophysics Vol. 317; no. 2; pp. 385 - 390
Main Authors Mascaro, C., Buesa, C., Ortiz, J.A., Hare, D., Hegardt, F.G.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 10.03.1995
Subjects
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Colon - enzymology
DNA, Complementary - chemistry
DNA, Complementary - isolation & purification
Gene Expression
Humans
Hydroxymethylglutaryl-CoA Synthase - genetics
Mitochondria, Liver - enzymology
Molecular Sequence Data
Organ Specificity
Rats
Restriction Mapping
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Summary:Mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase is a constituent of the HMG-CoA pathway responsible for ketone body synthesis and one of its main regulatory points. We report the isolation and characterization of a 2058-bp cDNA from human liver. This cDNA encodes a polypeptide of 508 residues and 56,635 Da. The homology with previously reported rat mitochondrial and human cytosolic HMG-CoA synthases is 88 and 66%, respectively. mRNA levels were high in liver and colon, low in testis, heart, skeletal muscle, and kidney, and faint in pancreas.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0003-9861
1096-0384
DOI:10.1006/abbi.1995.1178