Hydroethanolic extracts from different genotypes of açaí (Euterpe oleracea) presented antioxidant potential and protected human neuron-like cells (SH-SY5Y)

• Published in: Food chemistry Vol. 222; pp. 94 - 104
• Main Authors: Torma, Priscila do Carmo Marchioro Raupp, Brasil, Allana Von Sulzback, Carvalho, Ana Vânia, Jablonski, André, Rabelo, Thallita Kelly, Moreira, José Cláudio Fonseca, Gelain, Daniel Pens, Flôres, Simone Hickmann, Augusti, Paula Rossini, Rios, Alessandro de Oliveira
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2017
• Subjects: Anthocyanins; Antioxidant activity; Antioxidants - pharmacology; Breeding program; Carotenoids; Cell Line, Tumor; Dichlorofluorescein-diacetate assay; Euterpe - chemistry; Fruit; Genotype; Humans; Neurons - drug effects; Neuroprotective Agents - pharmacology; Plant Extracts - pharmacology; Reactive oxygen species; Reactive Oxygen Species - metabolism; Anthocyanins; Reactive oxygen species; Breeding program; Carotenoids; Dichlorofluorescein-diacetate assay; Antioxidant activity
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2016.12.006

•The breeding program has provided important changes in açaí bioactive compounds.•Cyanidin 3-glucoside and cyanidin 3-rutinoside are the main anthocyanins found.•Hydroethanolic extracts from açaí have a high content of carotenoids.•All genotypes, except L04P16, showed antioxidant and neuroprotective effects. Fruit breeding programs have resulted in bioactive compounds increase and health effects. Thus, this study aimed to evaluate the antioxidant activity and neuroprotective effects of the hydroethanolic extracts from six açaí (Euterpe oleracea) genotypes using ABTS, deoxyribose, and glutathione oxidation assays, as well as, SH-SY5Y cells insulted with H2O2. L22P13 genotype showed the highest total content of anthocyanins, while L06P13 showed a high content of total carotenoids. However, the genotypes showed no difference in the antioxidant activity by ABTS and deoxyribose assays. The hydroethanolic extracts from different genotypes of açaí showed a protective effect (13–62%) on SH-SY5Y cells insulted by H2O2 at a concentration of 50μg/mL by DCFH-DA assay. Except L04P16, no genotypes showed cytotoxicity in the SRB assay. These results indicate that açaí genotypes have antioxidant effect against reactive species generated in SH-SY5Y cells, suggesting a neuroprotective effect of the hydroethanolic extracts from these fruits.