Multilocus enzyme electrophoretic (MEE) analysis of Australian isolates of Salmonella Enteritidis

• Published in: International journal of food microbiology Vol. 31; no. 1; pp. 273 - 282
• Main Authors: Cox, J.M., Story, L., Bowles, R., Woolcock, J.B.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.08.1996; Elsevier
• Subjects: Alleles; AUSTRALIA; AUSTRALIE; Bacteriology; Biological and medical sciences; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; Electrophoresis, Polyacrylamide Gel - methods; Enzymes - genetics; EPIDEMIOLOGIA; EPIDEMIOLOGIE; EPIDEMIOLOGY; Food industries; Food Microbiology; Fundamental and applied biological sciences. Psychology; Genetic Variation; Microbiology; Multilocus enzyme electrophoresis; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Population structure; SALMONELLA ENTERITIDIS; Salmonella enteritidis - enzymology; Salmonella enteritidis - genetics; United States; Europe; America; United Kingdom; Australia; North America; Germany; Oceania; Population structure; Multilocus enzyme electrophoresis; Salmonella Enteritidis; Salmonella enteritidis; Genetic variability; Gel electrophoresis; Enzyme; Genetic distance; Genetic diversity; Epidemiology; Pathogenic; Bacteria; Isolate; Comparative study; Enterobacteriaceae; Polymorphism
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/0168-1605(96)01100-2

Seventy-three Australian isolates of Salmonella Enteritidis (SE) were analysed by multilocus enzyme electrophoresis (MEE) using a polyacrylamide gel system. Analysis of 11 enzyme loci identified eight electrophoretic types (ETs), with 61 of the isolates assigned to ET1, and 72 isolates considered to represent a clonal lineage. Representative isolates of each of the Australian ETs were then compared with isolates from England, Germany and the United States, using a starch gel system and 13 enzyme loci. The overseas isolates formed a single ET with representatives of the major Australian ET. It is concluded that Australian isolates of SE are closely related genetically to those from countries in which egg-borne transmission is common.