Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization

Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned...

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Bibliographic Details
Published inMolecular bioSystems Vol. 9; no. 4; pp. 626 - 629
Main Authors Chae, Pil Seok, Wander, Marc J, Cho, Kyung Ho, Laible, Philip D, Gellman, Samuel H
Format Journal Article
LanguageEnglish
Published England 05.04.2013
Subjects
Carbohydrates - chemistry
Detergents - chemistry
Membrane Proteins - chemistry
Micelles
Octoxynol - chemistry
Protein Stability
Solubility
Surface-Active Agents - chemistry
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Summary:Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.
ISSN:1742-206X
1742-2051
DOI:10.1039/c3mb25584k