Correlation Study on Expression of GST-π Protein in Brain Tissue and Peripheral Blood of Epilepsy Rats Induced by Pilocarpine

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 31; no. 5; pp. 701 - 704
• Main Author: 邓学军 贾红 杨志勇 李刚 孙圣刚
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.10.2011; Department of Neurology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022,China
• Subjects: Animals; Anticonvulsants - therapeutic use; Biomarkers; Brain - metabolism; Drug Resistance - physiology; Epilepsy - chemically induced; Epilepsy - drug therapy; Epilepsy - metabolism; Glutathione S-Transferase pi - blood; Glutathione S-Transferase pi - metabolism; Male; Medicine; Medicine & Public Health; Pilocarpine; Rats; 外周血; 大鼠模型; 抗癫痫; 消费税; 生理盐水; 脑组织; 药物反应; 蛋白质; epilepsy; pilocarpine; correlation study; GST-π protein; rats; brain
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-011-0586-x

Previous studies have suggested that glutathione-S-transferase π （GST-π） over-expression in the brain tissue is associated with refractory epilepsy. However, whether the change in GST-π level in the peripheral blood is in line with that in brain tissue remains unknown. This study examined the correlation between GST-π in brain tissue and that in peripheral blood in rat models of pilocarpine-induced refractory epilepsy. The animals were divided into drug-resistant group and drug-responsive group according to the response to anti-epileptic drugs. GST-π expression in brain tissue was immunohistochemically determined, while the expression of GST-π in peripheral blood was analyzed by Western blotting. In the hippocampus and cortex, GST-π was mainly found in the cytoplasm and membrane of neurons, and the GST-π expression level was higher in drug-resistant group than in the drug-responsive group and saline control group （P〈0.05）. Moreover, there was no significant difference between responders and saline control animals （P〉0.05）. The change in expression of GST-π in peripheral blood showed the same pattern as that in brain tissues, suggesting GST-π might contribute to drug resistance in epilepsy. Importantly, the GST-π over-expression in peripheral blood could be used as a marker for resistance to anti-epileptic agents.