Ensiled Mixed Vegetables Enriched Carbohydrate Metabolism in Heterofermentative Lactic Acid Bacteria

This study evaluated the fermentation quality, nutritive profile, in vitro fermentation, and microbial communities colonising sorghum ensiled with an unsalable vegetable mixture (chopped beans, carrot, and onion (1:1:1) ) including: (1)−100% sorghum; (2)−80% sorghum + 20% vegetable mix or (3)−60% so...

Full description

Saved in:
Bibliographic Details
Published inFermentation (Basel) Vol. 8; no. 12; p. 699
Main Authors Forwood, Daniel L, Holman, Devin B, Meale, Sarah J, Chaves, Alex V
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.12.2022
Subjects
16S rRNA sequencing
aerobic stability
Beans
Carbohydrate metabolism
Corn
Dry matter
Fatty acids
Fermentation
Glucosamine
Glycerol
Lactic acid bacteria
Lactobacillus
Nitrogen
Nutritional aspects
Observations
Physiological aspects
Probiotics
Proteins
rRNA 16S
silage microbial profile
Sorghum
Taxonomy
unsalable vegetable silage
Vegetables
Australia
United States > US
Online AccessGet full text

Cover

More Information
Summary:This study evaluated the fermentation quality, nutritive profile, in vitro fermentation, and microbial communities colonising sorghum ensiled with an unsalable vegetable mixture (chopped beans, carrot, and onion (1:1:1) ) including: (1)−100% sorghum; (2)−80% sorghum + 20% vegetable mix or (3)−60% sorghum + 40% vegetable mix, on a dry matter (DM) basis, with or without a probiotic inoculant. Samples were obtained across 0, 1, 3, 5,7, and 101 days ensiling and after 14 d aerobic exposure. The V4 region of the 16S rRNA gene and the ITS1 region were sequenced to profile bacterial, archaeal, and fungal communities. Compared to the 0% DM, ethanol increased (p < 0.01) from 8.42 to 20.4 ± 1.32 mM with 40% DM vegetable mix inclusion, while lactate decreased from 5.93 to 2.24 ± 0.26 mM. Linear discriminant analysis revealed that relative abundances of 12 bacterial taxa were influenced by silage treatments (log LDA score ≥ 4.02; p ≤ 0.03), while predicted functional pathways of alternative carbohydrate metabolism (hexitol, sulfoquinovose and glycerol degradation; N-acetyl glucosamine biosynthesis; log LDA score ≥ 2.04; p ≤ 0.02) were similarly enriched. This study indicated that carbohydrate metabolism by heterofermentative lactic acid bacteria can increase the feed value of sorghum when ensiled with an unsalable vegetable mixture at 40%DM, without requiring a high quantity of lactate.
ISSN:2311-5637
2311-5637
DOI:10.3390/fermentation8120699