The Expression of Truncated MK in Human Tumors

Midkine (MK) is a heparin binding growth/differentiation factor different from fibroblast growth factors (FGFs), is largely composed of two domains which are formed by a folded polypeptide chain interconnected by disulfide bridges. Polymerase chain reaction revealed the presence of a short MK mRNA i...

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Published inBiochemical and biophysical research communications Vol. 219; no. 1; pp. 256 - 260
Main Authors Kaname, Tadashi, Kadomatsu, Kenji, Aridome, Kuniaki, Yamashita, Shin-ichi, Sakamoto, Kiyoshi, Ogawa, Michio, Muramatsu, Takashi, Yamamura, Ken-ichi
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 06.02.1996
Subjects
Base Sequence
Blotting, Northern
Carrier Proteins - biosynthesis
Cell Line
Cytokines - biosynthesis
DNA Primers
DNA, Complementary
Exons
Gene Expression
Humans
Midkine
Molecular Sequence Data
Neoplasms - metabolism
Polymerase Chain Reaction
Reference Values
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
RNA, Neoplasm - analysis
RNA, Neoplasm - biosynthesis
Tumor Cells, Cultured
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Summary:Midkine (MK) is a heparin binding growth/differentiation factor different from fibroblast growth factors (FGFs), is largely composed of two domains which are formed by a folded polypeptide chain interconnected by disulfide bridges. Polymerase chain reaction revealed the presence of a short MK mRNA in 7 among 12 human tumor cells which expressed MK mRNA. All of 4 pancreatic carcinoma cell lines expressed the short species in addition to the full size mRNA. The short mRNA lacked an exon and resulted in the lack of a more N-terminally located domain. The truncated form of MK was found also in some surgically removed specimens of human tumors, but not in noncancerous tissue.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0214