Cyclooxygenase 2 Genetic Polymorphism May Increase the Risk of Developing Leukoaraiosis in Chinese

• Published in: Journal of molecular neuroscience Vol. 51; no. 2; pp. 461 - 466
• Main Authors: Shan, Xiao-yun, Chen, Guo-zhong, Cheng, Gan-ping, Tao, Hong-miao
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.10.2013; Springer Nature B.V
• Subjects: Adult; Aged; Alleles; Arachidonic acid; Biomedical and Life Sciences; Biomedicine; Case-Control Studies; Cell Biology; China; Cyclooxygenase 2 - genetics; Enzymes; Female; Genetic Predisposition to Disease; Haplotypes; Heterozygote; Hospitals; Humans; Inflammation; Leukoaraiosis - genetics; Linkage Disequilibrium; Male; Middle Aged; Neurochemistry; Neurology; Neurosciences; Pallets; Pathogenesis; Polymerase chain reaction; Polymorphism; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Proteomics; Stroke, Lacunar - genetics; China; Zhejiang China; Lacunar Infarction; Small Vessel Disease; Inflammation; Cyclooxygenase-2; Leukoaraiosis; Polymorphism; Linkage Disequilibrium
• ISSN: 0895-8696; 1559-1166
• DOI: 10.1007/s12031-013-0066-9

Cyclooxygenase-2 (COX-2) is a key enzyme involved in the conversion of arachidonic acid into prostaglandins, which are important mediators of inflammation. To clarify the role of inflammation in the pathogenesis of cerebral small vessel disease (SVD), we investigate the possible modulating effect of the functional COX-2 polymorphisms -1195G > A (rs689466) and -765G > C (rs20417) on the risk for development of cerebral SVD in a Chinese population. Genomic DNA of 116 patients with lacunar infarction (LI), 334 patients with leukoaraiosis (LA) and 450 control subjects was genotyped for the COX-2 -1195G > A and -765G > C polymorphisms using polymerase chain reaction-restriction fragment length polymorphism. Distribution of genotypes and haplotypes in patients and controls were compared. The genotype distribution of the -765G > C polymorphism was not different between the patients with LI or LA and the control group. The 1195A allele carriers was identified independently to be related with LA (adjusted OR = 1.41, 95 % confidence interval (CI) = 1.09–2.10, P  = 0.03) but not associated with LI. The linkage disequilibrium analysis showed that -1195G > A and -765G > C SNPs are moderate linkage disequilibrium in this study population ( D ′ = 0.70, r 2  = 0.16). Compared with G-1195-G-765 haplotype, the haplotype of A-1195-G-765 showed significantly increased the risk of LA (OR = 1.24, 95 % CI = 1.10–1.55, P  = 0.04) but not LI. In conclusion, we found that -1195G > A polymorphism and A-1195-G-765 haplotype of COX-2 were associated with susceptibility to LA in a Chinese population.