High-salt diet accelerates bone loss accompanied by activation of ion channels related to kidney and bone tissue in ovariectomized rats

• Published in: Ecotoxicology and environmental safety Vol. 244; p. 114024
• Main Authors: Cui, Yan, Sun, Kehuan, Xiao, Yawen, Li, Xiaoyun, Mo, Shu, Yuan, Yihan, Wang, Panpan, Yang, Li, Zhang, Ronghua, Zhu, Xiaofeng
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.10.2022; Elsevier
• Subjects: Bone metabolism; Epithelial sodium channel; High salt diet; Na-Cl cotransporter; Sodium calcium exchanger; Voltage-gated chloride channels; Voltage-gated chloride channels; Na-Cl cotransporter; High salt diet; Epithelial sodium channel; Bone metabolism; Sodium calcium exchanger
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2022.114024

Excessive salt intake can induce a variety of diseases, such as hypertension, cardiovascular disease, kidney disease and so on，it is also one of the factors promoting bone resorption. The mechanism of osteoporosis-induced exacerbations of high salt diet is not well-defined. In this study, we used ovariectomized 6-month-old Sprague Dawley rats to construct a high bone turnover model, and then administrated with high sodium chloride diet (2.0% w/w NaCl, 8.0% w/w NaCl) for 12 weeks to observe the effect of high salt diet on bone metabolism. The results showed that high salt diet could lead to the destruction of bone microstructure, promote the excretion of urinary calcium and phosphorus and accelerate the bone turnover, as well as cause the pathologic structural abnormalities in renal tubular. At the same time, it was accompanied by the up-regulated expression of the epithelial sodium channel (ENaCα), voltage-gated chloride channels (ClC)− 3 and the down-regulated expression of Na-Cl cotransporter (NCC), sodium calcium exchanger (NCX1) in femoral tissue and renal tubules. These findings confirm that high salt diet can destroy the microstructure of bone by increasing bone resorption and affect some ion channels of bone tissue and renal tubule in ovariectomized rats. •High salt diet exacerbates bone loss in ovariectomized rat.•High salt diet impairs bone microstructure in ovariectomized rat.•High salt diet affects the expression of the ion channels in bone tissue.