Localization of antigens and allergens in thin sections of the house dust mite, Dermatophagoides pteronyssinus (Acari: Pyroglyphidae)

Cryostat sections of the house dust mite, Dermatophagoides pteronyssinus, were probed in fluorescent microscopy studies with rabbit polyclonal and mouse monoclonal antibodies specific for mite allergens including the major allergen, Der p I. Sections also were probed for allergens with sera from hum...

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Bibliographic Details
Published inJournal of medical entomology Vol. 27; no. 3; p. 368
Main Authors Tovey, E.R. (Royal North Shore Hospital, St. Leonards, New South Wales, Australia), Baldo, B.A
Format Journal Article
LanguageEnglish
Published England 01.05.1990
Subjects
ALERGENOS
ALLERGENE
ALLERGENS
Allergens - analysis
ANATOMIA ANIMAL
ANATOMIE ANIMALE
ANIMAL ANATOMY
Animals
Antigens - analysis
Antigens, Dermatophagoides
Humans
INSECTE NUISIBLE
INSECTOS DANINOS
METHODE
METHODS
METODOS
MICROSCOPIA
MICROSCOPIE
MICROSCOPY
Mites - anatomy & histology
Mites - immunology
MORPHOLOGY
PEST INSECTS
SARCOPTIFORMES
STAINING
THIN SECTION PREPARATION
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Summary:Cryostat sections of the house dust mite, Dermatophagoides pteronyssinus, were probed in fluorescent microscopy studies with rabbit polyclonal and mouse monoclonal antibodies specific for mite allergens including the major allergen, Der p I. Sections also were probed for allergens with sera from human mite-allergic subjects containing IgE antibodies to mite allergens and with lectins. Antibody binding was mainly to the gut lining and gut contents of the mite, although some specific labeling also was associated with the head region and cuticle. This is the first detailed localization of mite allergens in situ. The morphology of the mite was investigated using plastic embedded thin sections and was found to be similar to that previously described for D. farinae.
Bibliography:9032093
L72
ISSN:0022-2585
1938-2928
DOI:10.1093/jmedent/27.3.368