Neurogenesis of Adipose-derived Stem Cells in Hydrogel

Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. I...

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Published inJournal of Huazhong University of Science and Technology. Medical sciences Vol. 31; no. 2; pp. 174 - 177
Main Author 谢雪微 唐洲平 陈娟 杨洁 曾文高 刘娜 刘永明
Format Journal Article
LanguageEnglish
Published Heidelberg Huazhong University of Science and Technology 01.04.2011
Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan 430030, China
Department of Neurology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China%Department of Neurology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China
Subjects
Adipose Tissue - cytology
Animals
Cell Differentiation - physiology
Hydrogels - chemistry
Male
Medicine
Medicine & Public Health
Neurons - cytology
Rats
Rats, Sprague-Dawley
Stem Cells - cytology
Tissue Engineering
Tissue Scaffolds
凝胶聚合物
医学应用
无性系种群
水凝胶
碱性成纤维细胞生长因子
神经再生
脂肪干细胞
表皮生长因子
adipose-derived stem cells
neurogenesis
tissue engineering
hydrogel
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Summary:Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells （ADSCs） were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal （NB） media supplemented with B27, basic fibroblast growth factor （bFGF） and epidermal growth factor （EGF）. Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.
Bibliography:Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells （ADSCs） were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal （NB） media supplemented with B27, basic fibroblast growth factor （bFGF） and epidermal growth factor （EGF）. Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.
42-1679/R
Xuewei XIE1, 2, Zhouping TANG1#, Juan CHEN1, Jie YANG1, Wengao ZENG1, Na LIU1, Yongming LIU1 1Department of Neurology, Tongji Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan 430030, China 2Department of Neurology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China
adipose-derived stem cells; hydrogel; neurogenesis; tissue engineering
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-011-0246-1