Advances in the metabolic engineering of Yarrowia lipolytica for the production of terpenoids

• Published in: Bioresource technology Vol. 281; pp. 449 - 456
• Main Authors: Ma, Yi-Rong, Wang, Kai-Feng, Wang, Wei-Jian, Ding, Ying, Shi, Tian-Qiong, Huang, He, Ji, Xiao-Jun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2019
• Subjects: Biological Products - metabolism; Metabolic engineering; Metabolic Engineering - methods; Mevalonate pathway; Terpenes - metabolism; Terpenoids; Yarrowia - metabolism; Yarrowia lipolytica; Metabolic engineering; Terpenoids; Mevalonate pathway; Yarrowia lipolytica
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2019.02.116

•Y. lipolytica is a promising chassis for the production of plant derived terpenoids.•Metabolic engineering strategies for terpenoids synthesis in Y. lipolytica are summarized.•Prospective for terpenoids synthesis in Y. lipolytica based on latest technology are discussed. Terpenoids are a large class of natural compounds based on the C5 isoprene unit, with many biological effects such activity against cancer and allergies, while some also have an agreeable aroma. Consequently, they have received extensive attention in the food, pharmaceutical and cosmetic fields. With the identification and analysis of the underlying natural product synthesis pathways, current microbial-based metabolic engineering approaches have yielded new strategies for the production of highly valuable terpenoids. Yarrowia lipolytica is a non-conventional oleaginous yeast that is rapidly emerging as a valuable host for the production of terpenoids due to its own endogenous mevalonate pathway and high oil production capacity. This review aims to summarize the status and strategies of metabolic engineering for the heterologous synthesis of terpenoids in Y. lipolytica in recent years and proposes new methods aiming towards further improvement of terpenoid production.