The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917

• Published in: Archives of microbiology Vol. 198; no. 7; pp. 603 - 610
• Main Authors: Yang, Ying, Yang, Yi, Ou, Bingming, Xia, Pengpeng, Zhou, Mingxu, Li, Luan, Zhu, Guoqiang
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2016; Springer Nature B.V
• Subjects: Amino acids; Animals; Bacteria; Bacterial Adhesion - genetics; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cell Line; E coli; Ecology; Escherichia coli; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - pathogenicity; Escherichia coli Infections - microbiology; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Female; Flagella - metabolism; Flagellin - genetics; Flagellin - metabolism; Gene Deletion; Genetic engineering; Life Sciences; Mice; Mice, Inbred BALB C; Microbial Ecology; Microbiology; Microscopy, Electron, Transmission; Original Paper; Peptides; Plasmids; Plasmids - genetics; Probiotics; Protein Structure, Tertiary; Salmonella; Swine; China; Flagellin; Flagella display application; strain Nissle 1917; E. coli strain Nissle 1917
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-016-1219-3

The most studied probiotic, Escherichia coli strain Nissle 1917 (EcN) possesses flagella of serotype H1. To explore the potential to use EcN flagellin in flagella display applications, we investigated the effect of deleting amino acids in the hypervariable region of flagellin on EcNc (EcN cured of its two cryptic plasmids pMUT1 and pMUT2). Two EcNc flagellin isogenic mutants with deletions of amino acid residual from 277 to 286 and from 287 to 296 in the hypervariable domain were constructed. Both mutants were flagellated, adherent to IPEC-J2 cells, and colonized BALB/c mice. These hypervariable regions may have future utility in the display of heterologous epitopes.