Murine Nr4a1 and Herpud1 are up-regulated by Wnt-1, but the homologous human genes are independent from beta-catenin activation

The Wnt signal transduction pathway regulates morphogenesis and mitogenesis of cells in multicellular organisms. A major downstream consequence of Wnt-1 signalling is the activation of beta-catenin/T-cell factor (TCF)-mediated transcription. We compared Wnt-1-transformed murine mammary epithelial ce...

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Published inBiochemical journal Vol. 367; no. Pt 3; pp. 723 - 728
Main Authors Chtarbova, Slava, Nimmrich, Inko, Erdmann, Silke, Herter, Peter, Renner, Matthias, Kitajewski, Jan, Müller, Oliver
Format Journal Article
LanguageEnglish
Published England 01.11.2002
Subjects
Animals
beta Catenin
Blotting, Northern
Cytoskeletal Proteins - metabolism
Humans
Immunohistochemistry
Mice
Proto-Oncogene Proteins - physiology
Signal Transduction
Trans-Activators - metabolism
Transcription, Genetic
Transfection
Up-Regulation - physiology
Wnt Proteins
Wnt1 Protein
Zebrafish Proteins
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Summary:The Wnt signal transduction pathway regulates morphogenesis and mitogenesis of cells in multicellular organisms. A major downstream consequence of Wnt-1 signalling is the activation of beta-catenin/T-cell factor (TCF)-mediated transcription. We compared Wnt-1-transformed murine mammary epithelial cells with control cells by subtractive hybridization. We found the two genes Nr4a1 and Herpud1 to be overexpressed in Wnt-1-transformed cells. Remarkably, the transcription levels of the two homologous human genes NR4A1 and HERPUD1 are neither activated in cells with activated beta-catenin/TCF-mediated transcription nor can be induced by beta-catenin transfection. These results indicate different regulation mechanisms of the two genes in murine and human cells.
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ISSN:0264-6021
1470-8728
DOI:10.1042/BJ20020699