Riparin B, a Synthetic Compound Analogue of Riparin, Inhibits the Systemic Inflammatory Response and Oxidative Stress in Mice

• Published in: Inflammation Vol. 38; no. 6; pp. 2203 - 2215
• Main Authors: Santiago, Renata Fortes, de Brito, Tarcisio Vieira, Dias, Jordana Maia, Dias, Genilson José, da Cruz, José Simião, Batista, Jalles Arruda, Silva, Renan Oliveira, Souza, Marcellus H. L. P., de Albuquerque Ribeiro, Ronaldo, Gutierrez, Stanley Juan Chavez, Freitas, Rivelilson M., Medeiros, Jand-Venes R., dos Reis Barbosa, André Luiz
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2015; Springer Nature B.V
• Subjects: Analgesics - pharmacology; Animals; Anti-Inflammatory Agents - pharmacology; Antioxidants - pharmacology; Benzamides - pharmacology; Biomedical and Life Sciences; Biomedicine; Carrageenan; Cytokines - immunology; Cytokines - metabolism; Disease Models, Animal; Edema - chemically induced; Edema - immunology; Edema - metabolism; Edema - prevention & control; Glutathione - metabolism; Immunology; Inflammation Mediators - immunology; Inflammation Mediators - metabolism; Internal Medicine; Male; Malondialdehyde - metabolism; Mice; Neutrophil Infiltration - drug effects; Nociceptive Pain - chemically induced; Nociceptive Pain - metabolism; Nociceptive Pain - prevention & control; Oxidative Stress - drug effects; Pathology; Peritonitis - chemically induced; Peritonitis - immunology; Peritonitis - metabolism; Peritonitis - prevention & control; Peroxidase - metabolism; Pharmacology/Toxicology; Phenethylamines - pharmacology; Rheumatology; Time Factors; anti-inflammatory; anti-oxidant; Riparin; anti-nociceptive
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-015-0203-4

The aim of our study was to evaluate the anti-inflammatory, anti-nociceptive, and anti-oxidant action of Riparin B in vivo . We performed experiments in which we induced paw edema by carrageenan and other mediators, carrageenan-induced peritonitis and the level of myeloperoxidase (MPO) activity, pro-inflammatory cytokines (TNF-α and IL-1β), malondialdehyde (MDA) acid, and glutathione (GSH) from the peritoneal fluid. We also performed behavior tests such as acetic acid-induced writhing, formalin-induced linking, and the hot plate test. Among the doses tested of the Riparin B (1, 3, and 10 mg/kg), the dose of 10 mg/kg showed the strongest effect, and this dose was able to reduce the paw edema induced by carrageenan, dextran, histamine serotonin, bradykinin, 48/80, and PGE2. Similarly, the Riparin B in the same dose reduced cell migration and significantly decreased the nociception induced by formalin and acetic acid and reversed the parameters of the oxidative stress. Thus, we can infer that Riparin B exhibits anti-inflammatory, anti-nociceptive, and anti-oxidant actions in vivo .